Title:
HIGHLY SPECIFIC GLYCOSYLTRANSFERASE FOR RHAMNOSE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/006109
Kind Code:
A1
Abstract:
Provided in the present invention are a highly specific glycosyltransferase for rhamnose, and the use thereof. A specific glycosyltransferase is disclosed in the present invention for the first time, which glycosyltransferase can catalyze rhamnosylation at a specific position of a substrate, and has high catalytic activity. Specifically, the specific glycosyltransferase of the present invention can specifically and efficiently catalyze the glycosylation of the C-6 position of a tetracyclic triterpene compound substrate on a first glycosyl so as to extend a rhamnose group. Also provided in the present invention is a mutant of the specific glycosyltransferase. The specific glycosyltransferase of the present invention has good specificity and high efficiency, can be applied to the construction of artificially synthesized ginsenoside, various new ginsenosides and derivatives thereof, and has good application value in the fields of pharmacy, etc.
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Inventors:
YAN XING (CN)
WANG PINGPING (CN)
LI CHAOJING (CN)
WANG PINGPING (CN)
LI CHAOJING (CN)
Application Number:
PCT/CN2022/109355
Publication Date:
February 02, 2023
Filing Date:
August 01, 2022
Export Citation:
Assignee:
SYNBIOTECH SUZHOU CO LTD (CN)
International Classes:
C12N9/10; C12N5/10; C12N15/54; C12N15/63; C12P33/00; C12P33/20
Foreign References:
| CN105177100A | 2015-12-23 | |||
| CN103849672A | 2014-06-11 | |||
| CN105985938A | 2016-10-05 | |||
| CN112831481A | 2021-05-25 | |||
| CN104232723A | 2014-12-24 | |||
| IL128193A | 2008-03-20 |
Other References:
LIANG HUI-CHAO, QING-HUA WANG, TING GONG, GUO-HUA DU, JIN-LING YANG, PING ZHU: "The basic strategies and research advances in the studies on glycosyltransferases involved in ginsenoside biosynthesis", ACTA PHARMACEUTICA SINICA, YAOXUE XUEBAO, CN, vol. 50, no. 2, 12 February 2015 (2015-02-12), CN , pages 148 - 153, XP093028469, ISSN: 0513-4870, DOI: 10.16438/j.0513-4870.2015.02.003
LI ZEDONG, ZHAO RONG-HUA; ZHANG ZHAO-CHUAN; YU JIE; GU WEN; HE SEN; CAO GUAN-HUA: "Advances in Biosynthesis and Ｒegulation Mechanism of Notoginseng Saponins", CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE, ZHONGGUO ZHONGYI KEXUEYUAN ZHONGYAO YANJIUSUO, CN, vol. 24, no. 14, 27 April 2018 (2018-04-27), CN , pages 207 - 213, XP093028467, ISSN: 1005-9903, DOI: 10.13422/j.cnki.syfjx.20181413
ZHAO JIA-NING, WANG RU-FENG, ZHAO SHU-JUAN, WANG ZHENG-TAO: "Advance in glycosyltransferases, the important bioparts for production of diversified ginsenosides", CHINESE JOURNAL OF NATURAL MEDICINES, ELSEVIER, AMSTERDAM, NL, vol. 18, no. 9, 1 September 2020 (2020-09-01), AMSTERDAM, NL, pages 643 - 658, XP093028466, ISSN: 1875-5364, DOI: 10.1016/S1875-5364(20)60003-6
QI XIAOQIANG, MA MING, WANG LAN, ZHANG YANG, JIANG RONG, BAI LIPING, LI YUAN: "Biochemical characterization of a novel bifunctional glycosyl-1-phosphate transferase involved in the exopolysaccharide biosynthesis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 465, no. 1, 1 September 2015 (2015-09-01), Amsterdam NL , pages 113 - 118, XP093028464, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2015.07.140
LI ZEDONG, ZHAO RONG-HUA; ZHANG ZHAO-CHUAN; YU JIE; GU WEN; HE SEN; CAO GUAN-HUA: "Advances in Biosynthesis and Ｒegulation Mechanism of Notoginseng Saponins", CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE, ZHONGGUO ZHONGYI KEXUEYUAN ZHONGYAO YANJIUSUO, CN, vol. 24, no. 14, 27 April 2018 (2018-04-27), CN , pages 207 - 213, XP093028467, ISSN: 1005-9903, DOI: 10.13422/j.cnki.syfjx.20181413
ZHAO JIA-NING, WANG RU-FENG, ZHAO SHU-JUAN, WANG ZHENG-TAO: "Advance in glycosyltransferases, the important bioparts for production of diversified ginsenosides", CHINESE JOURNAL OF NATURAL MEDICINES, ELSEVIER, AMSTERDAM, NL, vol. 18, no. 9, 1 September 2020 (2020-09-01), AMSTERDAM, NL, pages 643 - 658, XP093028466, ISSN: 1875-5364, DOI: 10.1016/S1875-5364(20)60003-6
QI XIAOQIANG, MA MING, WANG LAN, ZHANG YANG, JIANG RONG, BAI LIPING, LI YUAN: "Biochemical characterization of a novel bifunctional glycosyl-1-phosphate transferase involved in the exopolysaccharide biosynthesis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 465, no. 1, 1 September 2015 (2015-09-01), Amsterdam NL , pages 113 - 118, XP093028464, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2015.07.140
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SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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