Title:
GLYCOSYLTRANSFERASE OSUGT91C1 MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/083359
Kind Code:
A1
Abstract:
Provided are a glycosyltransferase OsUGT91C1 mutant and the use thereof. An amino acid sequence as shown in SEQ ID NO:. 1 is obtained by means of mutating an amino acid at position 208 from Phe to Met on the basis of the amino acid sequence of glycosyltransferase OsUGT91C1; and an amino acid sequence as shown in SEQ ID NO: 2 is obtained by means of mutating an amino acid at position 208 from Phe to Met and mutating an amino acid at position 379 from Phe to Ala on the basis of the amino acid sequence of glycosyltransferase OsUGT91C1, wherein the amino acids at positions 1-14 of the above-mentioned two mutants are redundant, and can be completely removed or changed without affecting normal enzyme activity. The mutant has an improved ability of catalyzing the same normal reaction, further removes the disadvantages of side reaction of the original OsUGT91C1 enzyme, and can catalyze the generation of a series of stevioside products including Reb E.
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Inventors:
ZHU XIAOFENG (CN)
Application Number:
PCT/CN2022/131774
Publication Date:
May 19, 2023
Filing Date:
November 14, 2022
Export Citation:
Assignee:
UNIV SICHUAN (CN)
International Classes:
C12N9/10; C12N1/21; C12N15/63; C12P33/20
Foreign References:
| CN114045273A | 2022-02-15 | |||
| US20150315623A1 | 2015-11-05 | |||
| CN113584016A | 2021-11-02 |
Other References:
DATABASE Protein NCBI; 7 August 2018 (2018-08-07), ANONYMOUS : "UDP-glycosyltransferase 91C1 [Oryza sativa Japonica Group]", XP093065284, Database accession no. XP_015629141.1
WANG, ZHENYANG ET AL.: "Heterologous expression of EUGT11 from Oryza sativa in Pichia pastoris for highly efficient one-pot production of rebaudioside D from rebaudioside A", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 163, 22 September 2020 (2020-09-22), XP086300466, DOI: 10.1016/j.ijbiomac.2020.09.132
HUANG XU-XU; ZHAO SHU-MAN; ZHANG YU-YING; LI YAN-JIE; SHEN HAN-NUO; LI XUGANG; HOU BING-KAI: "A novel UDP-glycosyltransferase 91C1 confers specific herbicide resistance through detoxification reaction in Arabidopsis", PLANT PHYSIOLOGY AND BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 159, 27 December 2020 (2020-12-27), AMSTERDAM, NL , pages 226 - 233, XP086468514, ISSN: 0981-9428, DOI: 10.1016/j.plaphy.2020.12.026
WANG, ZHENYANG ET AL.: "Heterologous expression of EUGT11 from Oryza sativa in Pichia pastoris for highly efficient one-pot production of rebaudioside D from rebaudioside A", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 163, 22 September 2020 (2020-09-22), XP086300466, DOI: 10.1016/j.ijbiomac.2020.09.132
HUANG XU-XU; ZHAO SHU-MAN; ZHANG YU-YING; LI YAN-JIE; SHEN HAN-NUO; LI XUGANG; HOU BING-KAI: "A novel UDP-glycosyltransferase 91C1 confers specific herbicide resistance through detoxification reaction in Arabidopsis", PLANT PHYSIOLOGY AND BIOCHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 159, 27 December 2020 (2020-12-27), AMSTERDAM, NL , pages 226 - 233, XP086468514, ISSN: 0981-9428, DOI: 10.1016/j.plaphy.2020.12.026
Attorney, Agent or Firm:
CHENGDU FANGYU UNION PATENT AGENCY (GENERAL PARTNERSHIP) (CN)
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