Title:
GENOME EDITING METHOD FOR DUPLICATED GENES
Document Type and Number:
WIPO Patent Application WO/2024/053677
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a technology for, by using a small amount of gRNA or crRNA, simultaneously editing numerous genes that are functionally duplicated. It was found that a CasΦ protein derived from huge phages can cut a target sequence having a nucleotide length of 16 or more, and a mismatch sequence including a mismatch of 1 or 2 bases with respect to the target sequence. Accordingly, provided is a genome editing method that is for duplicated genes and that uses said CasΦ protein.
Inventors:
SUGANO SHIGEO (JP)
HASEGAWA REIKA (JP)
HASEGAWA REIKA (JP)
Application Number:
PCT/JP2023/032542
Publication Date:
March 14, 2024
Filing Date:
September 06, 2023
Export Citation:
Assignee:
AIST (JP)
International Classes:
C12N15/09; C12N15/63
Domestic Patent References:
| WO2021236651A1 | 2021-11-25 | |||
| WO2021216512A1 | 2021-10-28 |
Other References:
PAUSCH PATRICK, AL-SHAYEB BASEM, BISOM-RAPP EZRA, TSUCHIDA CONNOR A., LI ZHENG, CRESS BRADY F., KNOTT GAVIN J., JACOBSEN STEVEN E.: "CRISPR-CasΦ from huge phages is a hypercompact genome editor", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 369, no. 6501, 17 July 2020 (2020-07-17), US , pages 333 - 337, XP055862891, ISSN: 0036-8075, DOI: 10.1126/science.abb1400
PAUSCH PATRICK; SOCZEK KATARZYNA M.; HERBST DOMINIK A.; TSUCHIDA CONNOR A.; AL-SHAYEB BASEM; BANFIELD JILLIAN F.; NOGALES EVA; DOU: "DNA interference states of the hypercompact CRISPR–CasΦ effector", NATURE STRUCTURAL & MOLECULAR BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 28, no. 8, 1 August 2021 (2021-08-01), New York , pages 652 - 661, XP037536569, ISSN: 1545-9993, DOI: 10.1038/s41594-021-00632-3
CAI QINAN, GUO DONGMEI, CAO YUJUN, LI YUAN, MA RUI, LIU WENPING: "Application of CRISPR/CasΦ2 System for Genome Editing in Plants", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 23, no. 10, 20 May 2022 (2022-05-20), Basel, CH , pages 5755, XP093147813, ISSN: 1422-0067, DOI: 10.3390/ijms23105755
ISHIHARA, RYOSUKE ET AL.: "Application of multiplex genome editing technology using RNA processing to plants", RECORDINGS OF RESEARCH PRESENTATIONS AT THE 80TH CONGRESS OF THE BOTANICAL SOCIETY OF JAPAN, P-1711, 2016, pages 241
PAUSCH PATRICK; SOCZEK KATARZYNA M.; HERBST DOMINIK A.; TSUCHIDA CONNOR A.; AL-SHAYEB BASEM; BANFIELD JILLIAN F.; NOGALES EVA; DOU: "DNA interference states of the hypercompact CRISPR–CasΦ effector", NATURE STRUCTURAL & MOLECULAR BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 28, no. 8, 1 August 2021 (2021-08-01), New York , pages 652 - 661, XP037536569, ISSN: 1545-9993, DOI: 10.1038/s41594-021-00632-3
CAI QINAN, GUO DONGMEI, CAO YUJUN, LI YUAN, MA RUI, LIU WENPING: "Application of CRISPR/CasΦ2 System for Genome Editing in Plants", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 23, no. 10, 20 May 2022 (2022-05-20), Basel, CH , pages 5755, XP093147813, ISSN: 1422-0067, DOI: 10.3390/ijms23105755
ISHIHARA, RYOSUKE ET AL.: "Application of multiplex genome editing technology using RNA processing to plants", RECORDINGS OF RESEARCH PRESENTATIONS AT THE 80TH CONGRESS OF THE BOTANICAL SOCIETY OF JAPAN, P-1711, 2016, pages 241
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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