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Title:
GENETIC SCISSORS-BASED RAPID DETECTION OF INFLUENZA VIRUS TYPE
Document Type and Number:
WIPO Patent Application WO/2022/177066
Kind Code:
A1
Abstract:
The present invention relates to a method and a kit for the genetic-scissors-based rapid detection of influenza virus type. Specifically, the present invention relates to: a molecular diagnosis method for rapidly detecting influenza virus type by using primer sets specific to each of influenza A virus (IAV) and influenza B virus (IBV), a guide RNA, and CRISPR-Cas12a; and a kit therefor.

Inventors:
SONG YOON-JAE (KR)
Application Number:
PCT/KR2021/005091
Publication Date:
August 25, 2022
Filing Date:
April 22, 2021
Export Citation:
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Assignee:
UNIV GACHON IND ACAD COOP FOUND (KR)
International Classes:
C12Q1/70; C12N9/22; C12N15/113
Foreign References:
CN111534643A2020-08-14
KR20190140918A2019-12-20
Other References:
FOAD MASHAYEKHI; RICKY Y. T. CHIU; ALEXANDER M. LE; FELIX C. CHAO; BENJAMIN M. WU; DANIEL T. KAMEI: "Enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 398, no. 7 - 8, 24 September 2010 (2010-09-24), Berlin, DE , pages 2955 - 2961, XP019863982, ISSN: 1618-2650, DOI: 10.1007/s00216-010-4213-7
JAVALKOTE VIVEK S., KANCHARLA NAGESH, BHADRA BHASKAR, SHUKLA MANISH, SONI BADRISH, SAPRE AJIT, GOODIN MICHAEL, BANDYOPADHYAY ANIND: "CRISPR-based assays for rapid detection of SARS-CoV-2", METHODS, ACADEMIC PRESS, NL, vol. 203, 1 July 2022 (2022-07-01), NL , pages 594 - 603, XP055960418, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2020.10.003
OSBORN MARK J., BHARDWAJ AKSHAY, BINGEA SAMUEL P., KNIPPING FRIEDERIKE, FESER COLBY J., LEES CHRISTOPHER J., COLLINS DANIEL P., ST: "CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics", BIOENGINEERING, vol. 8, no. 2, 1 February 2021 (2021-02-01), pages 23 - 20, XP055960421, ISSN: 2306-5354, DOI: 10.3390/bioengineering8020023
PARK BUM JU, PARK MAN SEONG, LEE JAE MYUN, SONG YOON JAE: "Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay", BIOSENSORS, M D P I AG, CH, vol. 11, no. 3, 1 March 2021 (2021-03-01), CH , pages 88 - 9, XP055960424, ISSN: 2079-6374, DOI: 10.3390/bios11030088
Attorney, Agent or Firm:
Y.P.LEE, MOCK & PARTNERS (KR)
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