FUSION PROTEIN, SUBSTANCE PRODUCTION METHOD, VECTOR, TRANSFORMED CELL, PNEUMATIC TIRE PRODUCTION METHOD, AND RUBBER PRODUCT PRODUCTION METHOD

Title:

FUSION PROTEIN, SUBSTANCE PRODUCTION METHOD, VECTOR, TRANSFORMED CELL, PNEUMATIC TIRE PRODUCTION METHOD, AND RUBBER PRODUCT PRODUCTION METHOD

Document Type and Number:

WIPO Patent Application WO/2021/010101

Kind Code:

A1

Abstract:

The present invention aims to provide: a fusion protein that has enzymatic activity for synthesizing a hydrophobic compound and can bind to oil droplets; a method for producing a substance that accumulates a hydrophobic compound inside an oil droplet, using the fusion protein; a vector that improves the productivity of the hydrophobic compound by being introduced to cells using genetic recombination technology; and transformed cells that have introduced thereto the vector or a gene that codes the fusion protein. This invention pertains to a fusion protein that has: an amino acid sequence (first amino acid sequence) that can bind to oil droplets (lipid droplets); and an amino acid sequence (second amino acid sequence) having an enzymatic activity that binds hydrophobic compounds. The first amino acid sequence and the second amino acid sequence fuse via a linker sequence (third amino acid sequence) and the enzymatic activity of the second amino acid sequence is maintained.

Inventors:

YAMAGUCHI HARUHIKO (JP)
INOUE YUKINO (JP)
TAKAHASHI SEIJI (JP)
NAKAYAMA TORU (JP)
YAMASHITA SATOSHI (JP)
TOZAWA YUZURU (JP)

Application Number:

PCT/JP2020/024525

Publication Date:

January 21, 2021

Filing Date:

June 23, 2020

Export Citation:

Click for automatic bibliography generation Help

Assignee:

SUMITOMO RUBBER IND (JP)
UNIV TOHOKU (JP)
UNIV KANAZAWA (JP)
NATIONAL UNIV CORPORATION SAITAMA UNIV (JP)

International Classes:

C12N15/62; C07K14/415; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12N15/29; C12N15/54; C12N15/63; C12P5/00

Domestic Patent References:

WO1996021029A1	1996-07-11
WO2017002818A1	2017-01-05
WO2017002818A1	2017-01-05
WO1994000977A1	1994-01-20

Foreign References:

JP2004527227A	2004-09-09
JP2015097523A	2015-05-28
JP2015136296A	2015-07-30
JPS59140885A	1984-08-13
JPS6070080A	1985-04-20
JPS60251887A	1985-12-12
JPH11127025A	1999-05-11

Other References:

HORN, PATRICK J. ET AL.: "Identification of a new class of lipid droplet- associated proteins in plants", PLANT PHYSIOL., vol. 162, 2013, pages 1926 - 1936, XP055790297
RAHAMAN, GENOMICS, vol. 14, 2013
ASAWATRERATANAKUL ET AL., EUROPEANJOURNAL OF BIOCHEMISTRY, vol. 270, 2003, pages 4671 - 4680
POST ET AL., PLANT PHYSIOLOGY, vol. 158, 2012, pages 1406 - 1417
KARINE ET AL., BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1838, 2014, pages 287 - 299
SHOTA ENDO, BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1625, 2003, pages 291 - 295
MASAHIRO FUJIHASHI, PNAS, vol. 98, no. 8, 2001, pages 4337 - 4342
PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
KARLINALTSCHUL: "FASTA", METHODS ENZYMOL., vol. 183, no. 63, 1990
"Molecular Cloning", 2001
"Methods for General and Molecular Bacteriology", 1994, ASM PRESS
"Immunology methods manual", ACADEMIC PRESS, article "Molecular"
GIDDA ET AL., PLANT PHYSIOLOGY!, vol. 170, April 2016 (2016-04-01), pages 2052 - 2071
PRATT, J.M. ET AL.: "Hames", vol. 179-209, 1984, IRL PRESS, article "Transcription and Translation"
SPIRIN, A.S. ET AL., SCIENCE, vol. 242, 1988, pages 1162 - 1164
KIGAWA ET AL., 21ST ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
FUJIMURA ET AL., PLANT TISSUE CULTURE LETT., vol. 2, no. 74, 1995
SHILLITO ET AL., BIO/TECHNOLOGY, vol. 7, 1989, pages 581
VISSER ET AL., THEOR. APPL. GENET., vol. 78, 1989, pages 589
AKAMA ET AL., PLANT CELL REP., vol. 12, 1992, pages 7
"pEU-E01-His-TEV-MCS-N2 vector", CELLFREE SCIENCE, MATSUYAMA, JAPAN
NOBUSAWA ET AL.: "Department of Life Science and Technology, School of Life Science and Technology", vol. 3, 2017, TOKYO INSTITUTE OF TECHNOLOGY, article "Biological system"
"ENDEXT Technology", CELLFREE SCIENCE
See also references of EP 3992295A4