Title:
FUSION PROTEASE, FUSION EXPRESSION VECTOR, ENGINEERED BACTERIUM, AND METHOD FOR PRODUCING N-ACETYLNEURAMINIC ACID
Document Type and Number:
WIPO Patent Application WO/2024/036508
Kind Code:
A1
Abstract:
Provided are a fusion protease, a fusion expression vector, an engineered bacterium, and a method for producing N-acetylneuraminic acid. The fusion protease comprises N-acetyl-D-glucosamine 2-epimerase (AGE), N-acetylneuraminic acid aldolase (NAL), and a linker (GGGGS)6. The flexible linker (GGGGS) links two key enzymes, i.e., the AGE and the NAL, in whole-cell biosynthesis of N-acetylneuraminic acid, so that the formation of a polymer from the fusion protease is facilitated, the catalytic capability of the fusion protease is equivalent to that in a native free state, and the fusion protease can be used for catalytic synthesis of N-acetylneuraminic acid.
Inventors:
CHEN FENG (CN)
LAO YONGMIN (CN)
LAO YONGMIN (CN)
Application Number:
PCT/CN2022/113029
Publication Date:
February 22, 2024
Filing Date:
August 17, 2022
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
C07K14/195; C12N1/21; C12N15/60; C12N15/61; C12N15/63; C12P13/02
Foreign References:
| CN101979644A | 2011-02-23 | |||
| CN109971696A | 2019-07-05 | |||
| CN108285886A | 2018-07-17 | |||
| CN104561194A | 2015-04-29 | |||
| US20130266987A1 | 2013-10-10 | |||
| CN111411066A | 2020-07-14 |
Other References:
GAO XINYUE, ZHANG FEIFEI, WU MENG, WU ZHIXIN, SHANG GUANGDONG: "-neuraminic Acid Aldolase", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 67, no. 22, 5 June 2019 (2019-06-05), US , pages 6285 - 6291, XP093140092, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.9b01839
Attorney, Agent or Firm:
SHENZHEN ZHONGYI UNION INTELLECTUAL PROPERTY AGENCY CO.,LTD. (CN)
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