Title:
ENZYME WITH D-AMINO ACID SYNTHESIS ACTIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/077428
Kind Code:
A1
Abstract:
Provided is an enzyme with D-amino acid synthesis activity. The enzyme has an amino acid sequence as shown in SEQ ID NO: 10. Compared with an original strain before mutation, the enzyme has a higher catalytic activity for D-amino acid synthesis, a higher enzyme activity and a higher conversion rate of D-amino acid, and remains more than 76% active in the pH 4-9 range.
Inventors:
PI LI (CN)
HUANG ZHIHUA (CN)
ZHA LIYAN (CN)
SU HAIXIA (CN)
SUN YUE (CN)
LIN LICHUN (CN)
LI KUN (CN)
TANG PENG (CN)
WANG JIONG (CN)
CHEN LEI (CN)
LI XIAOBO (CN)
WEI ZIXIANG (CN)
XING PANPAN (CN)
LIU JIN (CN)
WANG XIAOMENG (CN)
ZHU CHENGJUN (CN)
ZHANG TING (CN)
HUANG ZHIHUA (CN)
ZHA LIYAN (CN)
SU HAIXIA (CN)
SUN YUE (CN)
LIN LICHUN (CN)
LI KUN (CN)
TANG PENG (CN)
WANG JIONG (CN)
CHEN LEI (CN)
LI XIAOBO (CN)
WEI ZIXIANG (CN)
XING PANPAN (CN)
LIU JIN (CN)
WANG XIAOMENG (CN)
ZHU CHENGJUN (CN)
ZHANG TING (CN)
Application Number:
PCT/CN2022/124244
Publication Date:
April 18, 2024
Filing Date:
October 10, 2022
Export Citation:
Assignee:
WUHAN GRAND HOYO CO LTD (CN)
International Classes:
C12N9/88; C12N1/21; C12N15/70; C12P13/04; C12R1/19
Foreign References:
| CN104073506A | 2014-10-01 | |||
| CN110272856A | 2019-09-24 | |||
| CN113583989A | 2021-11-02 | |||
| US20060009524A1 | 2006-01-12 | |||
| US20100068771A1 | 2010-03-18 |
Other References:
FANG BAISHAN: "Directed Evolution of Enzymes in Vitro (Ⅱ) Methods of Enzyme-Library Screening and Its Application", JOURNAL OF HUAQIAO UNIVERSITY(NATURAL SCIENCE), vol. 26, no. 2, 20 April 2005 (2005-04-20), pages 113 - 116, XP093157852
FESKO.K.: "Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities", APPL. MICROBIOL. BIOTECHNOL., vol. 100, 26 January 2016 (2016-01-26), XP035870732, DOI: 10.1007/s00253-015-7218-5
陈启佳等 (CHEN, QIJIA ET AL.): "苏氨酸醛缩酶的催化机理、分子改造及合成应用 (Catalytic Mechanism, Molecular Engineering and Applications of Threonine Aldolases)", 生物工程学报 (CHINESE JOURNAL OF BIOTECHNOLOGY), vol. 37, no. 12, 25 December 2021 (2021-12-25), XP093086443, DOI: 10.13345/j.cjb.210089
DATABASE Protein 13 December 2017 (2017-12-13), DOWNLOAD ANONYMOUS: "DSD1 family PLP-dependent enzyme [Bordetella petrii]", XP093157854, retrieved from NCBI Database accession no. WP_012247070.1
FESKO.K.: "Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities", APPL. MICROBIOL. BIOTECHNOL., vol. 100, 26 January 2016 (2016-01-26), XP035870732, DOI: 10.1007/s00253-015-7218-5
陈启佳等 (CHEN, QIJIA ET AL.): "苏氨酸醛缩酶的催化机理、分子改造及合成应用 (Catalytic Mechanism, Molecular Engineering and Applications of Threonine Aldolases)", 生物工程学报 (CHINESE JOURNAL OF BIOTECHNOLOGY), vol. 37, no. 12, 25 December 2021 (2021-12-25), XP093086443, DOI: 10.13345/j.cjb.210089
DATABASE Protein 13 December 2017 (2017-12-13), DOWNLOAD ANONYMOUS: "DSD1 family PLP-dependent enzyme [Bordetella petrii]", XP093157854, retrieved from NCBI Database accession no. WP_012247070.1
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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