Title:
ENZYME FOR SYNTHESIZING HYDROXYL ACETALDEHYDE AND/OR 1,3-DIHYDROXYACETONE BY CATALYZING FORMALDEHYDE, AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/153306
Kind Code:
A1
Abstract:
Disclosed in the present invention are an enzyme for synthesizing hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde, and applications thereof. In the present invention, by means of site-directed mutation of BFD, a mutant of the enzyme is found; and by means of the mutant of the enzyme, polymerization of the formaldehyde is implemented; in addition, by means of F/XPK, generation of acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone is implemented; with combination of phosphotransacetylase (Pta), a route from the formaldehyde to acetyl coenzyme A is achieved in three steps with the enzyme.
Inventors:
JIANG HUIFENG (CN)
LU XIAOYUN (CN)
LIU YUWAN (CN)
YANG YIQUN (CN)
LU LINA (CN)
YANG SHENG (CN)
GOU JUNRAN (CN)
LU XIAOYUN (CN)
LIU YUWAN (CN)
YANG YIQUN (CN)
LU LINA (CN)
YANG SHENG (CN)
GOU JUNRAN (CN)
Application Number:
PCT/CN2018/076466
Publication Date:
August 30, 2018
Filing Date:
February 12, 2018
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N9/00; C12N15/52; C12P7/24; C12P7/26; C12P9/00; C12P19/32
Domestic Patent References:
| WO2015144447A1 | 2015-10-01 |
Foreign References:
| CN106916794A | 2017-07-04 | |||
| CN105132400A | 2015-12-09 |
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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