ENZYME AGENT AND USE THEREOF - AMANO ENZYME INC

Title:

ENZYME AGENT AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2024/071388

Kind Code:

A1

Abstract:

The present invention addresses the problem of providing a novel enzyme agent containing an aminopeptidase that exhibits different substrate specificity from conventional products. The present invention relates to an enzyme agent having as an active ingredient an aminopeptidase comprising an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 8 or an amino acid sequence equivalent thereto. The present invention also relates to an enzyme agent containing as an active ingredient an aminopeptidase that is derived from Aspergillus nidulans or Aspergillus oryzae and that has the following physicochemical properties. (1) Optimum temperature: near 50°C. (2) Optimum pH: near 7-8. (3) Substrate specificity: having highest specificity for an L-alanine residue or an L-lysine residue.

Inventors:

SAKAI KIYOTA (JP)
SHIMIZU MOTOYUKI (JP)
FURUSAWA TERUMI (JP)
NOMURA RYO (JP)
KAMEYAMA AYANE (JP)
KATO MASASHI (JP)

Application Number:

PCT/JP2023/035670

Publication Date:

April 04, 2024

Filing Date:

September 29, 2023

Export Citation:

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Assignee:

AMANO ENZYME INC (JP)
UNIV MEIJO (JP)

International Classes:

C12N9/50; A23L5/00; A23L27/00; A23L27/20; A61K38/48; C12N9/62; C12N15/57

Domestic Patent References:

WO2004105503A1

2004-12-09

Other References:

DATABASE UniProtKB 31 October 2006 (2006-10-31), E. ANONYMOUS: "Hypothetical protein", XP093156559, Database accession no. Q5AWZ9
DATABASE Protein 4 April 2018 (2018-04-04), ANONYMOUS: "unnamed protein product [Aspergillus oryzae RIB40]", XP093156561, Database accession no. XP_001825798
YOUHEI YAMAGATA: "Proteolytic Enzymes of A. oryzae", KAGAKU TO SEIBUTSU, vol. 54, no. 2, 1 January 2016 (2016-01-01), pages 109 - 116, XP093156564, DOI: 10.1271/kagakutoseibutsu.54.109