Title:
DOUBLE STRANDED CIRCULAR DNA VECTOR, METHOD FOR PRODUCING LINEAR COVALENTLY CLOSED DNA, AND FUSION POLYPEPTIDE CONTAINING PROTELOMERASE AND ENDONUCLEASE
Document Type and Number:
WIPO Patent Application WO/2024/058155
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a new method for simply and efficiently producing linear covalently closed DNA. Provided is a double stranded circular DNA vector, wherein the double stranded circular DNA vector contains a protelomerase gene sequence that encodes a protelomerase or an active fragment thereof, an endonuclease gene sequence that encodes an endonuclease or an active fragment thereof, a pair of protelomerase recognition sequences for recognition by the protelomerase or active fragment thereof to cleave the vector, at least one endonuclease recognition sequence for recognition by the endonuclease or active fragment thereof to cleave the vector, and a nucleic acid sequence of interest; the protelomerase gene sequence, the endonuclease gene sequence, and the endonuclease recognition sequence in the double stranded circular DNA vector are placed within the same region between the pair of protelomerase recognition sequences; the nucleic acid sequence of interest is placed within another region between the pair of protelomerase recognition sequences; and the protelomerase gene sequence and endonuclease gene sequence are placed under the control of a promoter capable of controlling expression.
Inventors:
SHIGETA MITSUKI (JP)
OSHIMA KANJI (JP)
NISHI TERUYUKI (JP)
MAEDA HIROFUMI (JP)
MINAKUCHI KAZUNOBU (JP)
OSHIMA KANJI (JP)
NISHI TERUYUKI (JP)
MAEDA HIROFUMI (JP)
MINAKUCHI KAZUNOBU (JP)
Application Number:
PCT/JP2023/033127
Publication Date:
March 21, 2024
Filing Date:
September 12, 2023
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N15/63; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/12; C12N9/16; C12N15/10; C12N15/11; C12N15/54; C12N15/55; C12N15/62
Domestic Patent References:
| WO2022156188A1 | 2022-07-28 | |||
| WO2022264095A1 | 2022-12-22 |
Foreign References:
| JP2013535210A | 2013-09-12 |
Other References:
SHIRLEY WONG, ET AL.: "Production of Double-stranded DNA Ministrings", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 108, no. 53177, 29 February 2016 (2016-02-29), pages 1 - 2, XP055587725, DOI: 10.3791/53177
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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