DIPHOSPHOMEVALONATE DECARBOXYLASE VARIANT AND METHOD FOR MANUFACTURING OLEFIN COMPOUND USING SAME

Title:

DIPHOSPHOMEVALONATE DECARBOXYLASE VARIANT AND METHOD FOR MANUFACTURING OLEFIN COMPOUND USING SAME

Document Type and Number:

WIPO Patent Application WO/2017/022804

Kind Code:

A1

Abstract:

In order to provide a method whereby an olefin compound can be manufactured with high productivity and to provide an enzyme for use in the method, the inventors introduced variations involving amino acid substitution at various sites of diphosphomevalonate decarboxylase (MVD) and prepared numerous MVD variants. As a result of evaluating the variants for catalytic activity relating to production of an olefin compound such as isoprene, the inventors discovered that MVD in which threonine at position 209 is substituted with another amino acid has catalytic activity, and made it clear that MVD in which arginine at position 74 is substituted with another amino acid has even higher catalytic activity.

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Inventors:

ORISHIMO RYOKO (JP)
SHIRAI TOMOKAZU (JP)
TAKAHASHI KAZUHIRO (JP)
HIZA MISAO (JP)
TANABE YUSUKE (JP)

Application Number:

PCT/JP2016/072828

Publication Date:

February 09, 2017

Filing Date:

August 03, 2016

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Assignee:

RIKEN (JP)
ZEON CORP (JP)
YOKOHAMA RUBBER CO LTD (JP)

International Classes:

C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12P5/02

Domestic Patent References:

WO2014100726A2	2014-06-26
WO2015004211A2	2015-01-15
WO2015021045A2	2015-02-12

Foreign References:

JP2015519083A	2015-07-09
JP2015501660A	2015-01-19
JP2015501659A	2015-01-19

Other References:

GOGERTY DS ET AL.: "Formation of isobutene from 3-hydroxy-3-methylbutyrate by diphosphomevalonate decarboxylase.", APPL. ENVIRON. MICROBIOL., vol. 76, no. 24, 2010, pages 8004 - 8010, XP002680645
RYOKO ORISHIMO ET AL.: "Yuyo Koso no Kishitsu Tokuisei Kaihen to Bio-isoprene Seisan eno Oyo", PROCEEDINGS OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 2016, 5 March 2016 (2016-03-05), pages 2J003, XP009508887

Attorney, Agent or Firm:

CENTCREST IP ATTORNEYS (JP)

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