Title:
DECARBOXYLASE AND METHOD FOR PRODUCING UNSATURATED HYDROCARBON COMPOUND USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/022083
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method by which it is possible to produce, with high productivity, an unsaturated hydrocarbon compound such as butadiene, and an enzyme used in said method, wherein mutations associated with amino acid substitutions are introduced at various positions of ferulic acid decarboxylase, and numerous variants of said enzyme are prepared. In addition, with regard to the variants, as a result of assessing catalytic activity pertaining to the production of butadiene, it was discovered that the catalytic activity improves according to the fact that the amino acid at position 395 is glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, phenylalanine, or the like.
Inventors:
SHIRAI TOMOKAZU (JP)
MORI YUTARO (JP)
MORI YUTARO (JP)
Application Number:
PCT/JP2018/027729
Publication Date:
January 31, 2019
Filing Date:
July 24, 2018
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12N15/53; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12P5/00
Domestic Patent References:
| WO2013082264A1 | 2013-06-06 | |||
| WO2014202838A1 | 2014-12-24 | |||
| WO2012174439A2 | 2012-12-20 | |||
| WO2012106516A1 | 2012-08-09 | |||
| WO2017033965A1 | 2017-03-02 | |||
| WO2017033965A1 | 2017-03-02 |
Foreign References:
| JP2014030376A | 2014-02-20 | |||
| JP2015228804A | 2015-12-21 |
Other References:
ANONYMOUS: "Putative Ferulic acid decarboxylase 1 [Aspergillus calidoustus)", 15 October 2018 (2018-10-15), XP055665741, Retrieved from the Internet
ANONYMOUS: "5-carboxyvanillate decarboxylase [Sphingomonas paucimobilis", 4 September 2002 (2002-09-04), XP055665742, Retrieved from the Internet
ANONYMOUS: "UbiD family decarboxylase [Catenulispora acidiphila DSM 44928)", 1 January 2014 (2014-01-01), pages 1 - 2, XP055665747, Retrieved from the Internet
PAYNE KARL A. P. ET AL.: "New cofactor supports a,B-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition", NATURE, vol. 522, 2015, pages 497 - 501, XP055393569, DOI: 10.1038/nature14560
WEBER HEIKE E. ET AL.: "Requirement of a Functional Flavin Mononucleotide Prenyltransferase for the Activity of a Bacterial Decarboxylase in a Heterologous Muconic Acid Pathway in Saccharomyces cerevisiae", APPL. ENVIRON. MICROBIOL., vol. 83, no. 10, pages e03472 - 16
KARL A. P. PAYNE ET AL., NATURE, vol. 522, no. 7557, 25 June 2015 (2015-06-25), pages 497 - 501
KIYOSHI KUDO ET AL., JOURNAL OF THE JAPAN PETROLEUM INSTITUTE, vol. 38, no. 1, 13 July 1994 (1994-07-13), pages 48 - 51
KUNKEL, T. A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HO, S. N.HUNT, H. D.HORTON, R. M.PULLEN, J. K.PEASE, L. R., GENE, vol. 77, 1989, pages 51 - 59
KRUYER NS ET AL., CURR OPIN BIOTECHNOL., vol. 45, June 2017 (2017-06-01), pages 136 - 143
See also references of EP 3660156A4
ANONYMOUS: "5-carboxyvanillate decarboxylase [Sphingomonas paucimobilis", 4 September 2002 (2002-09-04), XP055665742, Retrieved from the Internet
ANONYMOUS: "UbiD family decarboxylase [Catenulispora acidiphila DSM 44928)", 1 January 2014 (2014-01-01), pages 1 - 2, XP055665747, Retrieved from the Internet
PAYNE KARL A. P. ET AL.: "New cofactor supports a,B-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition", NATURE, vol. 522, 2015, pages 497 - 501, XP055393569, DOI: 10.1038/nature14560
WEBER HEIKE E. ET AL.: "Requirement of a Functional Flavin Mononucleotide Prenyltransferase for the Activity of a Bacterial Decarboxylase in a Heterologous Muconic Acid Pathway in Saccharomyces cerevisiae", APPL. ENVIRON. MICROBIOL., vol. 83, no. 10, pages e03472 - 16
KARL A. P. PAYNE ET AL., NATURE, vol. 522, no. 7557, 25 June 2015 (2015-06-25), pages 497 - 501
KIYOSHI KUDO ET AL., JOURNAL OF THE JAPAN PETROLEUM INSTITUTE, vol. 38, no. 1, 13 July 1994 (1994-07-13), pages 48 - 51
KUNKEL, T. A., PROC NATL ACAD SCI USA, vol. 82, no. 2, 1985, pages 488 - 492
HO, S. N.HUNT, H. D.HORTON, R. M.PULLEN, J. K.PEASE, L. R., GENE, vol. 77, 1989, pages 51 - 59
KRUYER NS ET AL., CURR OPIN BIOTECHNOL., vol. 45, June 2017 (2017-06-01), pages 136 - 143
See also references of EP 3660156A4
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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