Title:
CHEMICAL-ENZYME COUPLING METHOD FOR SYNTHESIZING ERGOTHIONEINE
Document Type and Number:
WIPO Patent Application WO/2023/213276
Kind Code:
A1
Abstract:
Provided is a chemical-enzyme coupling method for synthesizing ergothioneine. The method comprises the steps of chemically synthesizing histidine betaine, and synthesizing ergothioneine from the histidine betaine by using Schizosaccharomyces pombe ergothioneine synthetases SPEGT1-tr M10 and SPEGT2 M3 after directed evolution.
Inventors:
LIN LIANG (CN)
LV LONG (CN)
JIANG YAN (CN)
WU YAMING (CN)
SONG SHUCHENG (CN)
SUN LILI (CN)
DING XIAORAN (CN)
LV LONG (CN)
JIANG YAN (CN)
WU YAMING (CN)
SONG SHUCHENG (CN)
SUN LILI (CN)
DING XIAORAN (CN)
Application Number:
PCT/CN2023/092114
Publication Date:
November 09, 2023
Filing Date:
May 04, 2023
Export Citation:
Assignee:
SHANGHAI INST ORGANIC CHEMISTRY CAS (CN)
International Classes:
C12N9/02; C12N15/53; C12P17/10
Foreign References:
| CN110607286A | 2019-12-24 | |||
| CN113234652A | 2021-08-10 | |||
| US20170321235A1 | 2017-11-09 |
Other References:
WANG LI, WANG YANG, LI JIANGHUA, DU GUOCHENG, KANG ZHEN: "Construction and optimization of ergothioneine-producing Escherichia coli", SHENG WU GONG CHENG XUE BAO = CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 38, no. 2, 25 February 2022 (2022-02-25), pages 796 - 806, XP093106210, DOI: 10.13345/j.cjb.210166
STEVEN A. VAN DER HOEK, BEHROOZ DARBANI, KAROLINA E. ZUGAJ, BALA KRISHNA PRABHALA, MATHIAS BERNFRIED BIRON, MILICA RANDELOVIC, JAC: "Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, 11 October 2019 (2019-10-11), XP055705666, DOI: 10.3389/fbioe.2019.00262
STEVEN A. VAN DER HOEK, BEHROOZ DARBANI, KAROLINA E. ZUGAJ, BALA KRISHNA PRABHALA, MATHIAS BERNFRIED BIRON, MILICA RANDELOVIC, JAC: "Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 7, 11 October 2019 (2019-10-11), XP055705666, DOI: 10.3389/fbioe.2019.00262
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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