CONCENTRATED GROWTH FACTOR WITH PHOTOBIOSTIMULATION

DESCRIPTION TECHNICAL FIELD

The invention is about a centrifuge device with photobiostimulation effect in order to obtain growth factors from the person's own blood to be used for the acceleration of wound healing and for the treatment of open and closed wounds of hard and soft tissues in all fields of medicine, dentistry and veterinary medicine.

PRIOR ART

Regenerative medicine and dentistry is the main aim of contemporary treatments. The need and willingness of replacing lost or damaged tissue parts is the most common therapeutic research field of modern medicine.

The best tissue stimulation is obtained by autogenous growth factors which increase regeneratioN. Several materials and techniques are used for this purpose, (such as PRP, PDGF, PRF and CGF). Up to date various systems have been developed for the concentration and preparation of these growth factors. These can be listed as:

· Current techniques:

• Fibrin adhesive (1 970)

• Platelet concentrate (cPRP 1 998)

• Platelet rich plasma (PRP, 1 998)

• Platelet rich growth factors(PRGF,1 998)

· Platelet rich fibrin (PRF, 2001 )

• Concentrated Growth Factors (CGF, 2006)

• Leukocyte and Platelet rich fibrin (L-PRF, 2006)

• Advanced platelet rich fibrin (A-PRF, 201 2)

• Injectable platelet rich fibrin (l-PRF, 2013)

However, none of these systems have been proven to be exactly successful in terms of biostimulation. The reason for this can be that these systems cannot precisely reveal the regenerative potential of whole blood.

Another approach for the biostimulation of cells or tissues to achieve regeneration is the photobiostimulation technique. Photobiostimulation, which is also called as low level laser therapy, is a photobiological technique in which the laser beam is applied to live tissues in milliwatts in order to accelerate wound healing.

The indications of regenerative techniques include acceleration of wound healing, esthetic and reconstructive surgery, delaying or decreasing the effects of aging, transplantation of hair, dermatologic and orthopedic indications in medicine; the treatment gingival and periodontal diseases, closure of gingival recessions, treatment of periimplantitis, mouth and jaw diseases and surgery and again wound healing acceleration in dentistry.

BRIEF DESCRIPTION OF THE INVENTION

The regenerative use of growth factors derived from the person's own blood has been popular in medical and dental fields in recent years. However, current techniques have limited effects due to the incapability of revealing full regenerative potential of blood. This invention can express the full regenerative capacity of the blood with the addition of LED light having photobiostimulation effect into the conventional techniques.

The invention is about a centrifuge device for the separation of shaped elements of blood to accelerate the time and quality of wound healing, the centrifuging of the blood containing tubes in various period, speed and angles in order to obtain platelet concentrates and growth factors, application of LED light in required appropriate wavelengths for biostimulation and preserving optimal temperature for the viability of cells. LIST OF FIGURES

Figure 1 . Upper View of the Centrifuge Device for Platelet-Rich Fibrin and

Concentrated Growth Factor with Photobiostimulation Figure 2. Centrifuge Container and Centrifuge Tubes The corresponding parts of the numbers given in the figures are:

LED Source

2 External rotor electrical motor

3 Microprocessor

4 Centrifuge Container Infrared heater

Cooler

Container DETAILED DESCRIPTION OF THE INVENTION

Obtaining growth factors from patient's own blood and photobiostimulation with LED light at appropriate wavelengths are two separate techniques used fort he stimulation for cell proliferation and subsequent wound healing acceleration. The combination of these two techniques which have positive effects on soft and hard tissues in a single, novel and original device will provide even more benefit.

Growth factors at various concentrations are obtained from the centrifuging of patient's own blood at varying time period, speed and angles with the aim of application to the wound area to increase the time and quality of healing. The centrifugal procedure is based on centralized forces solely and results in a limited amount of growth factors. The application of LED light which have photobiostimulation effect at appropriate wavelengths simultaneously during the centrifugal procedure (between 1 -30 minutes) will increase the amount of growth factors and the viability of the cells. During the centrifugal procedures, the blood is manipulated outside of the body, therefore the heating or cooling control of the device will provide optimal temperature for the cells.

The device which has a microprocessor for controlling the motor providing the centrifuging of the blood filled tube at various time period, speed and angle (also to be connected to PC, tablet and cell phones via wireless data transmission) will additionally apply LED light to with photobiostimulation effect in order to increase the amount of growth factors and viability of cells.

This invention increases the regenerative potential of the blood and biostimulation of related cells and tissues by additional LED light at appropriate wavelengths together with CGF (concentrated growth factors) and PRF (Platelet rich fibrin). The centrifuge designed to obtain CGF and PRF with photobiostimulation effect is composed of LED source (1 ) to apply light at appropriate wavelengths which has photobiostimulation effect, external rotor electrical motor (2), Microprocessor (3) for the control of external rotor electrical motor and LED source (1 ) which is capable of wireless data transmission and control of the system and its software which is included in motor driver, the centrifuge container (4) in which at least on tube can placed at adjustable and standardized angles, an infrared heater (5) to heat the inner cabinet, cooler (6) to cool the inner container, heat sensor for the control of inner cabinet, container (7) to place the LED source (1 ) which can apply light at appropriate wavelengths for photobiostimulation, touchscreen dashboard for displaying program information and control panel providing the control of the procedure.

The LED source (1 ) is the piece which can apply light at appropriate wavelengths for photobiostimulation. It can be used for applying light at 1 00-2000nm wavelength for biostimulation and at low level diode laser characteristics. The power and the application time can be controlled by the microprocessor.

External rotor electrical motor (2) can turn clockwise or anti-clockwise according to the selected program and protocol between 1 0-1 0000 rpm in terms of speed and time period.

The microprocessor (3) controls the external rotor motor (2) and LED source (1 ). This is the part that can enable wireless data transmission and wireless control; and can adjust the speed, timing and angulation of the rotation of the external electrical engine according to the user- chosen program and protocol, determine; and can determine the application time (1 -60 minutes) and power of the LED source (1 ) which has photobiostimulation effect at appropriate wavelength; and can control the heaters and coolers which adjusts the inner cabinet temperature until it reaches the desired degree during centrifuging procedure and waiting period and can stabilize desired temperature.

The software of the microprocessor (3) which controls the external rotor electrical motor (2) and LED source (1 ), can enable wireless data transmission and wireless control; and can adjust the speed, timing and angulation of the rotation of the external electrical engine according to the user- chosen program and protocol, determine; and can determine the application time (1 -60 minutes) and power of the LED source which has photobiostimulation effect at appropriate wavelength; and can control the heaters and coolers which adjusts the inner cabinet temperature until it reaches the desired degree during centrifuging procedure and waiting period and can stabilize desired temperature; and can digitally store patient information.

The motor driver is controlled by the microprocessor (3) and this part provides the required cycles, speed, timing and angulation and required centrifuging direction of the external rotor electrical engine according to the user-chosen program and protocol.

The centrifuge container (4) is directly connected to and can move simultaneously with the external rotor of the external rotor electrical motor (2); has constant or adjustable angulated form to place at least one blood tube. In addition, the Led sources which have photobiostimulation effect at appropriate wavelengths are placed into the centrifuge container in order to apply direct photobiostimulation to the blood tubes.

The infrared heater (5) which provides heating of the inner cabinet can increase the temperature of the tubes and the cabinet before and during the procedure and during the waiting period according to the user-chosen program and protocol under the control of the microprocessor (3).

The cooler (6) which provides cooling of the inner cabinet can decrease the temperature of the tubes and the cabinet before and during the procedure and during the waiting period according to the user-chosen program and protocol under the control of the microprocessor (3).

The heat sensor which controls the overall temperature adjusts the temperature of the tubes and the cabinet before and during the procedure and during the waiting period according to the user-chosen program and protocol under the control of the microprocessor (3) in case of a decrease or an increase of the temperature, it commands the microprocessor (3) to provide the required temperature.

The cabinet (7) in which the LED source (1 ) that applies light with photobiostimulation effects at appropriate wavelengths is placed has a structure with required amount and angulated LED sources can be placed in order to achieve LED light which has photobiostimulation effect on the blood tube before and during the centrifuging procedure and during the waiting period.

The touch-screen dashboard is located on the cabinet (7) and shows the program information. Before and during the procedure and during the waiting period the screen visually displays the procedure information and cabinet temperature.

The control panel is also located on the cabinet (7) together with the touchscreen dashboard and this part provides the user to select the centrifuge and photobiostimulation procedure parameters according to the user-chosen program and protocol to command the microprocessor.