Title:
CAPTURE-THEN-RELEASE MICROBIAL METHOD AND SYSTEM THAT ACCUMULATE AND RELEASE PLASTIC PARTICLES
Document Type and Number:
WIPO Patent Application WO/2024/078463
Kind Code:
A1
Abstract:
Provided is a microbial system established based on a genetically modified bacterial strain under a genus of Pseudomonas with improved capacities to form more biofilms and trap more microplastics from a source than its wild-type form. In particular, the genetically modified bacterial strain is selected from P. aeruginosa, and the genetically modified P. aeruginosa is provided by adaptive mutation to a gene responsible for regulating an adhesion protein expression in an exopolymeric matrix of the biofilms. A method for recovering the microplastics trapped by the biofilms of this genetically modified bacterial strain is also provided.
Inventors:
CHUA SONG LIN (CN)
LIU YANG (CN)
LIU YANG (CN)
Application Number:
PCT/CN2023/123611
Publication Date:
April 18, 2024
Filing Date:
October 09, 2023
Export Citation:
Assignee:
UNIV HONG KONG POLYTECHNIC (CN)
International Classes:
C12N1/20; C02F3/34; C12R1/385
Domestic Patent References:
| WO2019245986A1 | 2019-12-26 |
Foreign References:
| CN114058558A | 2022-02-18 |
Other References:
SHEPHERD YUEN CHAN: "Microbial–Enzymatic Combinatorial Approach to Capture and Release Microplastics", ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS, AMERICAN CHEMICAL SOCIETY, US, vol. 9, no. 11, 8 November 2022 (2022-11-08), US , pages 975 - 982, XP093158043, ISSN: 2328-8930, DOI: 10.1021/acs.estlett.2c00558
LIU, S.Y. ET AL: "Engineering a microbial ‘trap and release’ mechanism for microplastics removal", CHEMICAL ENGINEERING JOURNAL, vol. 404, 23 September 2020 (2020-09-23), XP086326258, DOI: 10.1016/j.cej.2020.127079
PUREVDASH TSEND AYUSH: "Characteristics of Initial Attachment and Biofilm Formation of Pseudomonas aeruginosa on Microplastic Surfaces", APPLIED SCIENCES, MDPI SWITZERLAND, vol. 12, no. 10, 1 January 2022 (2022-01-01), pages 5245, XP093158044, ISSN: 2076-3417, DOI: 10.3390/app12105245
SHANSHAN LI: "Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 5, no. 1, 23 November 2015 (2015-11-23), US , pages 16915, XP093158046, ISSN: 2045-2322, DOI: 10.1038/srep16915
PU, M. ; WOOD, T.K.: "Tyrosine phosphatase TpbA controls rugose colony formation in Pseudomonas aeruginosa by dephosphorylating diguanylate cyclase TpbB", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 402, no. 2, 12 November 2010 (2010-11-12), Amsterdam NL , pages 351 - 355, XP027487736, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2010.10.032
LIU, S.Y. ET AL: "Engineering a microbial ‘trap and release’ mechanism for microplastics removal", CHEMICAL ENGINEERING JOURNAL, vol. 404, 23 September 2020 (2020-09-23), XP086326258, DOI: 10.1016/j.cej.2020.127079
PUREVDASH TSEND AYUSH: "Characteristics of Initial Attachment and Biofilm Formation of Pseudomonas aeruginosa on Microplastic Surfaces", APPLIED SCIENCES, MDPI SWITZERLAND, vol. 12, no. 10, 1 January 2022 (2022-01-01), pages 5245, XP093158044, ISSN: 2076-3417, DOI: 10.3390/app12105245
SHANSHAN LI: "Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 5, no. 1, 23 November 2015 (2015-11-23), US , pages 16915, XP093158046, ISSN: 2045-2322, DOI: 10.1038/srep16915
PU, M. ; WOOD, T.K.: "Tyrosine phosphatase TpbA controls rugose colony formation in Pseudomonas aeruginosa by dephosphorylating diguanylate cyclase TpbB", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 402, no. 2, 12 November 2010 (2010-11-12), Amsterdam NL , pages 351 - 355, XP027487736, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2010.10.032
Attorney, Agent or Firm:
BEIJING PAT SF INTELLECTUAL PROPERTY AGENCY CO. LTD (CN)
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