Title:
CAMP FLUORESCENT PROBES G-FLAMP2 AND G-FLAMP2B AND USE THEREOF, AND KIT
Document Type and Number:
WIPO Patent Application WO/2024/077672
Kind Code:
A1
Abstract:
Provided are cAMP green fluorescent probes G-Flamp2 and G-Flamp2b and the use thereof, and a kit. The cAMP fluorescent probe G-Flamp2 has an amino acid sequence as shown in SEQ ID NO: 1; and the other cAMP fluorescent probe G-Flamp2b has an amino acid sequence as shown in SEQ ID NO: 2. The advantages comprise: (1) the probes have wide detection ranges; (2) the probes can be used in the detection of cAMP signals in living cells and living animal bodies; (3) the probes can be used for drug screening; and (4) the probes can be used in combination with fluorescent probes of other colors or photosensitive protein tools.
Inventors:
CHU JUN (CN)
WANG LIANG (CN)
LIU WENFENG (CN)
WANG LIANG (CN)
LIU WENFENG (CN)
Application Number:
PCT/CN2022/128591
Publication Date:
April 18, 2024
Filing Date:
October 31, 2022
Export Citation:
Assignee:
SHENZHEN INST ADV TECH (CN)
International Classes:
C07K19/00; C12N15/62; G01N21/64
Foreign References:
| CN113567402A | 2021-10-29 | |||
| CN113024674A | 2021-06-25 | |||
| CN109627344A | 2019-04-16 | |||
| US20070111270A1 | 2007-05-17 |
Other References:
JIAYUAN ZHANG; YUXIN SONG; CHUCHEN WANG; JING WANG: "A Novel Fluorescent Sensor for cAMP Based on E. Coli CAP Protein", JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES, SCHOOL OF PHARMACEUTICAL SCIENCES, BEIJING MEDICAL UNIVERSITY, BEIJING , CN, vol. 28, no. 8, 31 December 2019 (2019-12-31), Beijing , CN , pages 556 - 560, XP009553838, ISSN: 1003-1057
SEIKO KAWATAA: "Green Fluorescent cAMP Indicator of High Speed and Specificity Suitable for Neuronal Live-cell Imaging", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 119, no. 28, 6 July 2022 (2022-07-06), pages e2122618119, XP093157583, ISSN: 0027-8424
SEIKO KAWATAA: "Green Fluorescent cAMP Indicator of High Speed and Specificity Suitable for Neuronal Live-cell Imaging", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 119, no. 28, 6 July 2022 (2022-07-06), pages e2122618119, XP093157583, ISSN: 0027-8424
Attorney, Agent or Firm:
MING & YUE INTELLECTUAL PROPERTY LAW FIRM (CN)
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