Title:
BIOLOGICAL SUBSTANCE TREATMENT METHOD, REACTION DETECTION METHOD, BIOLOGICAL SUBSTANCE TREATMENT APPARATUS, AND REACTION DETECTION APPARATUS
Document Type and Number:
WIPO Patent Application WO/2024/085132
Kind Code:
A1
Abstract:
This biological substance treatment method involves preparing a flow cell 1 comprising: a chamber array device 11 provided with a plurality of chambers 14 in which one of the chambers is 1 nanoliter or less; and a cover body 21 defining a flow path 40 that is provided so as to be in communication with an opening of the chamber 14 and shared by the plurality of chambers 14. Next, prepared are DEX and PEG, which are both water-soluble and phase-separate when left to stand at room temperature. The property of DEX is that DEX phase-separates into the chamber 14 and organic substances are preferentially distributed to DEX more than PEG. Then, the organic substances are mixed in PEG. DEX is allowed to flow in the flow path 40 so that DEX is filled in the chamber 14 and in the flow path 40. Next, PEG is allowed to flow in the flow path 40, and as a result, DEX, which is remaining there, is pushed out and DEX and PEG come into contact with each other. Consequently, the organic substances are concentrated in DEX in the chamber 14.
Inventors:
NOJI HIROYUKI (JP)
MINAGAWA YOSHIHIRO (JP)
MINAGAWA YOSHIHIRO (JP)
Application Number:
PCT/JP2023/037489
Publication Date:
April 25, 2024
Filing Date:
October 17, 2023
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12Q1/6806; C12M1/00; C12M1/34; C12N9/22; C12N15/10; C12Q1/34; C12Q1/6876; G01N1/10; G01N1/40
Other References:
KAZUHITO V. TABATA, YOSHIHIRO MINAGAWA, YUKO KAWAGUCHI, MANA ONO, YOSHIKI MORIIZUMI, SEIYA YAMAYOSHI, YOICHIRO FUJIOKA, YUSUKE OHB: "Antibody-free digital influenza virus counting based on neuraminidase activity", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 December 2019 (2019-12-01), XP055676628, DOI: 10.1038/s41598-018-37994-6
ARLYNE B. SIMON: "Aqueous two-phase systems enable multiplexing of homogeneous immunoassays", TECHNOLOGY, WORLD SCIENTIFIC PUBLISHING CO., SI, vol. 02, no. 02, 1 June 2014 (2014-06-01), SI , pages 176 - 184, XP093160555, ISSN: 2339-5478, DOI: 10.1142/S2339547814500150
NAOKI NAKATANI; HIROKI SAKUTA; MASAHITO HAYASHI; SHUNSUKE TANAKA; KINGO TAKIGUCHI; KANTA TSUMOTO; KENICHI YOSHIKAWA: "Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self‐Emergence of a Cell‐Like Structure", CHEMBIOCHEM, JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 19, no. 13, 1 June 2018 (2018-06-01), Hoboken, USA, pages 1370 - 1374, XP072198957, ISSN: 1439-4227, DOI: 10.1002/cbic.201800066
MARCOS K. MASUKAWA: "Water‐in‐Water Droplets Selectively Uptake Self‐Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology", CHEMBIOCHEM, JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 23, no. 17, 5 September 2022 (2022-09-05), Hoboken, USA, XP093160557, ISSN: 1439-4227, DOI: 10.1002/cbic.202200240
ABRIL TORRES-BAUTISTA: "Characterization and optimization of polymer-polymer aqueous two-phase systems for the isolation and purification of CaCo2 cell-derived exosomes", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 17, no. 9, 2 September 2022 (2022-09-02), US , pages e0273243, XP093160563, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0273243
ARLYNE B. SIMON: "Aqueous two-phase systems enable multiplexing of homogeneous immunoassays", TECHNOLOGY, WORLD SCIENTIFIC PUBLISHING CO., SI, vol. 02, no. 02, 1 June 2014 (2014-06-01), SI , pages 176 - 184, XP093160555, ISSN: 2339-5478, DOI: 10.1142/S2339547814500150
NAOKI NAKATANI; HIROKI SAKUTA; MASAHITO HAYASHI; SHUNSUKE TANAKA; KINGO TAKIGUCHI; KANTA TSUMOTO; KENICHI YOSHIKAWA: "Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self‐Emergence of a Cell‐Like Structure", CHEMBIOCHEM, JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 19, no. 13, 1 June 2018 (2018-06-01), Hoboken, USA, pages 1370 - 1374, XP072198957, ISSN: 1439-4227, DOI: 10.1002/cbic.201800066
MARCOS K. MASUKAWA: "Water‐in‐Water Droplets Selectively Uptake Self‐Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology", CHEMBIOCHEM, JOHN WILEY & SONS, INC., HOBOKEN, USA, vol. 23, no. 17, 5 September 2022 (2022-09-05), Hoboken, USA, XP093160557, ISSN: 1439-4227, DOI: 10.1002/cbic.202200240
ABRIL TORRES-BAUTISTA: "Characterization and optimization of polymer-polymer aqueous two-phase systems for the isolation and purification of CaCo2 cell-derived exosomes", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 17, no. 9, 2 September 2022 (2022-09-02), US , pages e0273243, XP093160563, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0273243
Attorney, Agent or Firm:
KISARAGI ASSOCIATES (JP)
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