Title:
05SAR-PAGE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/221182
Kind Code:
A1
Abstract:
Provided are a 05SAR-PAGE, a preparation method therefor, and an application thereof, relating to a gel electrophoresis separation technique. The 05SAR-PAGE is a gel prepared from sodium lauroyl sarcosinate with a mass-volume ratio of 0.05%, and comprises a lower separation gel and an upper concentration gel. The lengths and thicknesses of the lower separation gel and the upper concentration gel are the same, and the ratio of the height of the lower separation gel to the height of the upper concentration gel is equal to 2:1 to 8:1. The present invention resolves the shortcomings in which SDS-PAGE cannot be used to analyze physiological state protein modification and native-PAGE cannot use protein markers for calibration and cannot be directly applied to the analysis of acidic and basic proteins. The present invention has universality, simple operation, and low costs, and is widely applicable to fundamental and applied research in the fields of molecular dynamics, enzymology, medicine, etc., bringing extensive convenience to the scientific analysis of proteins for experimenters.
Inventors:
HUANG LIQUN (CN)
JIANG LING (CN)
LI CONGGANG (CN)
JIANG LING (CN)
LI CONGGANG (CN)
Application Number:
PCT/CN2020/087126
Publication Date:
November 05, 2020
Filing Date:
April 27, 2020
Export Citation:
Assignee:
INNOVATION ACADEMY FOR PRECISION MEASUREMENT SCIENCE AND TECH CHINESE ACADEMY OF SCIENCES (CN)
International Classes:
G01N27/447; B01D57/02; G01N33/68
Foreign References:
| CN110133091A | 2019-08-16 | |||
| CN106885778A | 2017-06-23 | |||
| CN105842238A | 2016-08-10 | |||
| CN109609524A | 2019-04-12 | |||
| CN108717125A | 2018-10-30 |
Other References:
THIBEAULT, JANE ET AL.: "Sarkosyl: A Milder Detergent than SDS for Identifying Proteins with Moderately High Hyperstability Using Gel Electrophoresis", ANALYTICAL BIOCHEMISTRY, 16 February 2019 (2019-02-16), XP055750353, ISSN: 0003-2697, DOI: 202
Attorney, Agent or Firm:
WUHAN GENUIRY INTELLECTUAL PROPERTY FIRM (GENERAL PARTNER) (CN)
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