Title:
【発明の名称】pcl遺伝子の発現によりペニシリウム・クリソゲヌムにおけるペニシリンG(ベンジルペニシリン)の生産性を高めるための方法
Document Type and Number:
Japanese Patent JPH11506025
Kind Code:
A
Abstract:
Process for increasing production of penicillin G (benzylpenicillin) in Penicillium chrysogenum by expression of the pcl gene. The pcl gene codes for the enzyme phenylacetyl-CoA ligase. The pcl gene obtained from Pseudomonas putida U, and characterized by the sequence SEQ ID NO: 1, is introduced by means of recombinant DNA techniques, giving transformed CECT 20192 mutants, which show an increase of at least 10% in production of penicillin G relative to the untransformed control strains.
Inventors:
Minan Breath Rodriguez, Balthasar
Martinez Blanco, Honorina
Rodriguez Olivera, Elias
Garcia Alonso, Belem
Fernandez Canyon, Jose Manuel
Barredo Fuente, Jose Luis
Des Garcia, Bruno
Sleisner Sanchez, Carmen
Moreno Valle, Miguel Angel
Salt Maldonado, Francisco
Ruengo Rodriguez, Jose Maria
Martinez Blanco, Honorina
Rodriguez Olivera, Elias
Garcia Alonso, Belem
Fernandez Canyon, Jose Manuel
Barredo Fuente, Jose Luis
Des Garcia, Bruno
Sleisner Sanchez, Carmen
Moreno Valle, Miguel Angel
Salt Maldonado, Francisco
Ruengo Rodriguez, Jose Maria
Application Number:
JP53316897A
Publication Date:
June 02, 1999
Filing Date:
March 18, 1997
Export Citation:
Assignee:
Antibiotics, Socieda Anonima
International Classes:
C12N15/09; C12N1/15; C12N1/21; C12N9/00; C12N15/00; C12P37/02; C12R1/40; C12R1/66; C12R1/82; C12R1/865; (IPC1-7): C12N15/09; C12N1/15; C12N1/21; C12N9/00; C12P37/02
Attorney, Agent or Firm:
Takashi Ishida (3 others)