Title:
【発明の名称】D-アミノ酸オキシダ-ゼ及びその単離方法
Document Type and Number:
Japanese Patent JPS62501677
Kind Code:
A
Abstract:
A Trigonopsis variabilis D-amino acid oxidase in substantially pure form and active against cephalosporin C. This D-amino acid oxidase is isolated from Trigonopsis variabilis by a method which is performed in three steps, namely: (a) acidifying and heating a crude cell extract of Trigonopsis variabilis to obtain a precipitate and supernatant fraction; (b) treating said supernatant fraction obtained in step (a) with sufficient ammonium sulfate to obtain a second precipitate, said second precipitate containing the D-amino acid oxidase of claim 1; and (c) resuspending the precipitate obtained in step (b) and collecting the D-amino acid oxidase by isoelectric precipitation.
Inventors:
Moss Butch, Claus
Suwazawa Acres, Estella
Suwazawa Acres, Estella
Application Number:
JP50058286A
Publication Date:
July 09, 1987
Filing Date:
January 10, 1986
Export Citation:
Assignee:
Moss Butch, Claus
International Classes:
C12N9/06; C12N11/10; C12P35/00; C12R1/645; (IPC1-7): C12N9/06; C12N11/10; C12P35/00; C12R1/645
Domestic Patent References:
| JPS507158A | 1975-01-24 |
Attorney, Agent or Firm:
Takehiko Suzue