Title:
RNA干渉を媒介する短鎖RNA分子
Document Type and Number:
Japanese Patent JP4095895
Kind Code:
B2
Abstract:
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
Inventors:
Toushur, Thomas
Elvasher, Seida
Rendeckel, winfried
Wilm, Matthias
Ruhrmann, Reinhard
Elvasher, Seida
Rendeckel, winfried
Wilm, Matthias
Ruhrmann, Reinhard
Application Number:
JP2002546670A
Publication Date:
June 04, 2008
Filing Date:
November 29, 2001
Export Citation:
Assignee:
Max-Plank-Gezelle Shaft Tool Forderung Del Wissenschafften A.V.
Europay She Laboratorium Fur Molecular Biology (EMB)
Europay She Laboratorium Fur Molecular Biology (EMB)
International Classes:
A01H5/00; A01K67/027; C12N15/09; A61K31/7105; A61K45/00; A61K48/00; A61P31/12; A61P35/00; A61P37/02; A61P43/00; C07H21/00; C12N1/00; C12N5/10; C12N15/10; C12N15/113; C12P21/02; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/68; A61K9/00; A61K38/00; C07H; C12N
Foreign References:
| WO2001075164A1 |
Other References:
Genes & Dev., 15(2001.01.15) p.188-200
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Ishii Teiji
Satoshi Fujita
Ishii Teiji