Title:
PRODUCTION OF 7-AMINOCEPHEM COMPOUND OR ITS SALTS
Document Type and Number:
Japanese Patent JP3239359
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a new DNA fragment comprising a DNA fragment having a promoter active part of an alkali protease gene of Acremonium chrysogenum and useful for production, etc., of 7-aminocephem compound for antibiotic raw materials.
SOLUTION: This new DNA fragment has a promoter active part of an alkali protease gene of Acremonium chrysogenum. A 7-aminocephem compound can be obtained in high yield by introducing a vector constructed by binding a gene encoding cephalosporin C acylase represented by amino acid sequence 1-773 represented by the formula into cephalosporin compound-producing bacterium belonging to Acremonium chrysogenum to transform the vector and culturing the bacterium. The DNA fragment is obtained by probing chromosome DNA library of Acremoium chrysogenum with a partial sequence of an alkali protease gene and screening a promoter activity of the resultant gene.
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Inventors:
Takao Isogai
Masao Fukagawa
Morita Iwami
Ichiro Aramori
Takajo Jin
Masao Fukagawa
Morita Iwami
Ichiro Aramori
Takajo Jin
Application Number:
JP2000002148A
Publication Date:
December 17, 2001
Filing Date:
November 30, 1990
Export Citation:
Assignee:
Fujisawa Pharmaceutical Co., Ltd.
International Classes:
C12N1/15; C12N15/09; C12N1/21; C12N9/06; C12N9/58; C12N9/80; C12N15/55; C12N15/57; C12N15/80; C12P17/00; C12P17/18; C12P35/02; C12R1/01; C12R1/645; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Eiji Tabuse