Title:
PROCESS FOR ENZYMATIC PREPARATION OF 7-AMINO-CEPHALOSPORANIC ACID
Document Type and Number:
Japanese Patent JP3195058
Kind Code:
B2
Abstract:
PURPOSE: To efficiently obtain the subject compound useful for a raw material of an antibacterial agent, etc., without using any toxic solvent or reagent by selectively deacylating 7-acylamino-cephalosporanic acid with a microorganism belonging to genus Pseudomonas, or an enzyme derived from its product.
CONSTITUTION: This 7-amino-cephalosporanic acid expressed by formula II is obtained under a modest condition by subjecting a compound of formula I [R is H, OH or O-CO-R" (R" is a 1-4C alkyl); R' is COOH] to a selective deacylating reaction in the presence of a microorganism selected from the group consisting of Pseudomonas, Bacillus, Achromobacter genera (e.g. Achromobacber xylosoxidans 13/D7, NCIMB40407) or in the presence of an enzyme, either free or immobilized, derived therefrom, at 0.1-2% concentration and 20-40°C temperature.
Inventors:
Ezio Battister
Pietro Chesti
Juliana Franzossi
Wilhelms van der Hoose
Pietro Chesti
Juliana Franzossi
Wilhelms van der Hoose
Application Number:
JP19836392A
Publication Date:
August 06, 2001
Filing Date:
July 24, 1992
Export Citation:
Assignee:
Ministero del Universita et del la Ricierca Scientificeca e Technoca
International Classes:
C12N1/20; C12P35/02; C12P35/00; C12R1/025; C12R1/07; C12R1/38; (IPC1-7): C12P35/02; C12N1/20
Attorney, Agent or Firm:
Minoru Nakamura (6 outside)