Title:
電気穿孔法及び微量注入法を組み合わせた脂質二重膜の貫通方法
Document Type and Number:
Japanese Patent JP4755388
Kind Code:
B2
Abstract:
Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed between said needle-shaped object, with the tip of said needle-shaped object placed in contact with said conainer in such a way that it applies a mechanical force to the lipid membrane of said container, thus mechanically straining it, and a second electrode, whereupon a transient electric pulse of 1-to-103 V/cm is applied between the electrodes, resulting in a focused electrical field over said container C which induces a dielectric breakdown of the lipid bilayer causing the needle-shaped object to penetrate the container. Disclosed is also an electroinjection method based on the above method, wherein substances are introduced through the needle-shaped object and into the container after penetration of the needle-shaped object.
Inventors:
Olwar, Owe
Carlson, Matthias
Chu, daniel
Carlson, Matthias
Chu, daniel
Application Number:
JP2002536436A
Publication Date:
August 24, 2011
Filing Date:
October 19, 2001
Export Citation:
Assignee:
Select Recon Arb
International Classes:
C12N13/00; C12N15/09; B01L3/02; C12N15/89
Domestic Patent References:
| JP8322548A |
Foreign References:
| WO1999024110A1 |
Attorney, Agent or Firm:
Haruo Hamada