Title:
【発明の名称】アフィニティークロマトグラフィー用のポリマー性担体を活性化する方法およびそれらから製造された組成物
Document Type and Number:
Japanese Patent JPH05504978
Kind Code:
A
Abstract:
A novel class of compounds, methods for the preparation thereof and the use thereof in chromatographic methods for binding various biologically active materials non-covalently are disclosed. The class of compounds comprises the reaction product of a polymeric gel with a pyridine base, such as 4-dimethylaminopyridine (DMAP), and a halogen-substituted pyridine, such as 3,5-dichloro-2,4,6-trifluoropyridine (DCTFP), which reaction product may in turn be optionally reacted with hydroxyl ions or specified low-molecular-weight compounds. These compounds are capable of selectively and efficiently binding proteins and other organic materials of interest non-covalently to a degree comparable or superior to the heretofore preferred natural affinity ligands, such as Protein A gels. The novel compounds find particular utility in purification and recovery of proteins such as serum albumin and immunoglobulins of various classes from crude sources, such as diluted serum samples from various species.
Inventors:
Ngo, That Tea
Application Number:
JP50076490A
Publication Date:
July 29, 1993
Filing Date:
November 27, 1989
Export Citation:
Assignee:
Unisin Fibertech Corporation
International Classes:
A61K39/395; B01D15/08; B01J20/281; B01J20/32; C07K1/22; C07K14/765; C07K16/06; C08B37/00; C08F8/30; A61K39/00; C08G65/32; C08G69/00; C08G69/48; C12N9/00; C12N11/06; C12N11/08; G01N30/88; G01N33/531; (IPC1-7): A61K39/00; A61K39/395; C08B37/00; C08F8/30; C08G65/32; C08G69/00; C12N11/08; G01N30/48; G01N33/531
Attorney, Agent or Firm:
Kyozo Yuasa (5 people outside)