Title:
機能改変されたコレステロールオキシダーゼ
Document Type and Number:
Japanese Patent JP4008263
Kind Code:
B2
Abstract:
To realize an oxidation activity for the degradation of cholesterol and a new function related with the synthesis of a useful substance and associated with the degradation of the cholesterol.
A function-modified cholesterol oxidase in which serine as an amino acid at the position 379 (pretype) or position 337 (mature type) of a cholesterol oxidase as a cholesterol-decomposing enzyme originating from Streptomyces, is replaced with a hydrophobic amino acid has an oxidation activity against the cholesterol and a high oxidation activity against pregrenolone.
COPYRIGHT: (C)2003,JPO
Inventors:
Murooka Yoshikatsu
Mitsuo Yamashita
Mitsuo Yamashita
Application Number:
JP2002056574A
Publication Date:
November 14, 2007
Filing Date:
March 01, 2002
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
C12N9/04; C12N15/09; C12P33/02; C12R1/465
Domestic Patent References:
| JP2001029091A |
Foreign References:
| WO1999045106A1 | ||||
| WO1993007261A1 |
Other References:
日本生物工学会大会講演要旨集、1996年、第1996巻、225頁、750
Attorney, Agent or Firm:
Toshio Nishizawa