Title:
【発明の名称】小容積体を制御操作する方法及び微小流体デバイス
Document Type and Number:
Japanese Patent JP2003507162
Kind Code:
A
Abstract:
A method for conducting a broad range of biochemical analyses or manipulatio ns on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemente d on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses a nd manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channe l device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
Inventors:
Ramsey, Jay Michael
Jacobson, Stefan, Cie.
Jacobson, Stefan, Cie.
Application Number:
JP2001516663A
Publication Date:
February 25, 2003
Filing Date:
August 10, 2000
Export Citation:
Assignee:
Utility-Battle, LRC
International Classes:
B01D57/02; B01F5/04; B01F13/00; B01J4/00; B01J19/00; B01J19/32; G01N27/447; B01L3/00; B03C5/00; F15C5/00; F16K99/00; G01N37/00; G11C19/00; G11C25/00; G01N35/08; (IPC1-7): B01J4/00; B01D57/02; B01J19/32; B03C5/00; G01N27/447; G01N37/00
Attorney, Agent or Firm:
Kazuo Takeshita