Title:
アミダーゼ、それをコードする核酸、および、それを作製および使用する方法
Document Type and Number:
Japanese Patent JP2005525102
Kind Code:
A
Abstract:
This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7-aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(epsilon-D-2-aminoadipoyladipoylamido)-4-methylcoumarin.
Inventors:
Burton Nelson Earl
Weiner David Paul
Greenberg william
Lusa mantha
Chan Christine
Waters Elizabeth
Weiner David Paul
Greenberg william
Lusa mantha
Chan Christine
Waters Elizabeth
Application Number:
JP2003564209A
Publication Date:
August 25, 2005
Filing Date:
January 28, 2003
Export Citation:
Assignee:
Diversa Corporation
International Classes:
A01K67/027; A23C19/032; A23C19/06; A61K38/00; A61P31/04; A61P31/10; C07K14/00; C07K16/40; C07K19/00; C12M1/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/78; C12N9/80; C12N9/86; C12N15/09; C12P21/08; C12P35/00; C12P35/02; A01H5/00; C12Q1/34; G01N33/53; G01N37/00; (IPC1-7): C12N15/09; A01H5/00; A01K67/027; A23C19/032; A23C19/06; A61K38/00; A61P31/04; A61P31/10; C07K14/00; C07K16/40; C07K19/00; C12M1/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/80; C12N9/86; C12P21/08; G01N33/53; G01N37/00
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama