Title:
７デヒドロコレステロール及びビタミンＤ３の製造法
Document Type and Number:
Japanese Patent JP6533747
Kind Code:
B2
Abstract:
According to the present invention, there can be provided A process for producing 7-dehydrocholesterol (hereinafter, "7DHC"), comprising culturing, in a medium, a 7DHC-producing Labyrinthulea microorganism in which the 7DHC reducing activity is reduced or lost as compared to a parent strain through deletion, substitution, or addition of at least one base in a gene which is present in the chromosomal DNA of the parent strain and encodes a protein having 7DHC reducing activity, and the microorganism produces 7DHC, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, the 7DHC produced by the production process.
Inventors:
Tetsuro Ushihara
Satoshi Mitsuhashi
Satoshi Mitsuhashi
Application Number:
JP2015557843A
Publication Date:
June 19, 2019
Filing Date:
January 14, 2015
Export Citation:
Assignee:
Kyowa Hakko Bio Co., Ltd.
International Classes:
C12P33/00; C07C401/00; C12N15/53
Domestic Patent References:
| JP2000026405A |
Foreign References:
| US20060240508 |
Other References:
WATERHAM H. R. et al.,Smith-Lemli-Opitz syndrome is caused by mutations in the 7-dehydrocholesterol reductase gene,Am. J. Hum. Genet.,1998年,vol.63, no.2,p.329-338
HUANG J. et al.,Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid,J. Biotechnol.,2008年,vol.138, no.1-2,p.9-16
HUANG J. et al.,Expressed sequence tag analysis of marine fungus Schizochytrium producing docosahexaenoic acid,J. Biotechnol.,2008年,vol.138, no.1-2,p.9-16
Attorney, Agent or Firm:
Patent business corporation glory patent office