Title:
YAXAB NANOPORE, NANOPORE SYSTEM COMPRISING SAME AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/090998
Kind Code:
A1
Abstract:
The present invention relates to a YaxAB nanopore, a system comprising the nanopore and applications thereof. The YaxAB nanopore of the present invention has a funnel-shaped three-dimensional structure so that a change in current blocking according to movement of an analyte captured inside the nanopore can be maximized to enable very sensitive measurements of even the structure of the analyte and minute physical and chemical characteristic changes including dynamic change and the like, and can cause very strong electroosmosis with high cation selectivity such that various types of analytes can be captured and analyzed without limiting the types of overall charges. Additionally, with respect to the nanopore of the present invention, the amino acid sequence of a monomer constituting the nanopore can be adjusted to improve protein purification yield and control oligomerization mechanism, and thus a YaxAB nanopore having a pore size that cannot be formed by a wild-type monomer can be prepared. Furthermore, with respect to the nanopore of the present invention, YaxAB nanopores of various pore sizes can be ensured using one-time purification, and a nanopore of a desired pore size can be selectively ensured. Therefore, limitations of a conventional biological nanopore with a fixed pore size can be overcome such that, with respect to the YaxAB nanopore of the present invention, the size of a nanopore can be adjusted according to the size of an analyte. Additionally, the YaxAB nanopore of the present invention has analyte analysis spectrums of a very wide dynamic range of detection from a small analyte up to a large analyte, resulting from the funnel-shaped three-dimensional structure with a wide inlet and a narrow outlet. Moreover, the YaxAB nanopore of the present invention has the largest nanopore structure among biological nanopores up to now, and large-sized analytes that cannot be detected by conventional biological nanopores can undergo single molecule detection and analysis on the basis of a funnel structure with a large inlet. In addition, the present invention has established an optimized composition of a membrane in which the YaxAB nanopore can exist stably, and, by using a sensor implemented on the basis thereof, various types of analytes having relatively various sizes and charges can be qualitatively and quantitatively analyzed at a single molecular level with very high resolution and sensitivity.
Inventors:
CHI SEUNG-WOOK (KR)
JEONG KI BAEK (KR)
KIM JIN SIK (KR)
LEE MI-KYUNG (KR)
RYU MINJU (KR)
OH SO HEE (KR)
CHUNG MINJI (KR)
JO JUNHYEOK (KR)
JEONG KI BAEK (KR)
KIM JIN SIK (KR)
LEE MI-KYUNG (KR)
RYU MINJU (KR)
OH SO HEE (KR)
CHUNG MINJI (KR)
JO JUNHYEOK (KR)
Application Number:
PCT/KR2023/016681
Publication Date:
May 02, 2024
Filing Date:
October 25, 2023
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
G01N33/487; C07K14/00
Attorney, Agent or Firm:
BAE, KIM & LEE IP (KR)
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