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Title:
THREE-DIMENSIONAL TISSUE CULTURE AND METHOD FOR PRODUCING THE SAME
Document Type and Number:
WIPO Patent Application WO/2024/085257
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a three-dimensional tissue culture and a method for producing the same. The problem is solved by this method for producing a three-dimensional tissue culture, the method comprising: a step for seeding first cells on a culture substrate, applying a centrifugal force, and subsequently culturing the cells to form a first cell layer; and a step for seeding second cells on the first cell layer, applying a centrifugal force, and subsequently culturing the cells to laminate a second cell layer.

Inventors:
MURAHASHI MUTSUNORI (JP)
NISHIKAWA MAKIYA (JP)
KUSAMORI KOUSUKE (JP)
OBANA SHU (JP)
Application Number:
PCT/JP2023/038104
Publication Date:
April 25, 2024
Filing Date:
October 20, 2023
Export Citation:
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Assignee:
THE JIKEI UNIV (JP)
UNIV TOKYO SCIENCE FOUND (JP)
International Classes:
C12N5/071; A61L27/38; C12N5/0775
Domestic Patent References:
WO2022070787A12022-04-07
WO2016031824A12016-03-03
WO2005107642A12005-11-17
Foreign References:
JP2019010030A2019-01-24
Other References:
YOSHIYA ASANO, DAISUKE OKANO, MICHIYA MATSUSAKI, TETSURO WATABE, YASUHIRO YOSHIMATSU, MITSURU AKASHI, HIROSHI SHIMODA : "Construction of transplantable artificial vascular tissue based on adipose tissue-derived mesenchymal stromal cells by a cell coating and cryopreservation technique", SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, US, vol. 11, no. 1, US , XP093162182, ISSN: 2045-2322, DOI: 10.1038/s41598-021-97547-2
OBANA, SHU ET AL.: "Development of three-dimensional lymphatic network visceral tissue based on laminated cell sheet fabrication technology using centrifugal force", THE 22ND CONGRESS OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE, 23 March 2023 (2023-03-23), pages 276
OBANA, SHU ET AL.: "Lymph node regeneration by lymphatic network visceral tissue transplantation created using centrifugal force", THE 39TH ANNUAL MEETING OF THE JAPAN SOCIETY OF DRUG DELIVERY SYSTEM, 10 July 2023 (2023-07-10), pages 160
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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