Title:
STREPTOVARICIN DERIVATIVE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/029408
Kind Code:
A1
Abstract:
Provided are a streptovaricin derivative, a preparation method therefor, and an application thereof, pertaining to the field of bio-pharmaceuticals. The novel streptovaricin derivative 1-4 is obtained by using Streptomyces spectabilis XW-1 (accession number: CCTCC NO: M2017417) as a starting strain, and carrying out single inactivation or deletion on P450 genes (svP4 and stvP5) of a post-modification system in a streptovaricins biosynthesis gene cluster of the strain, followed by carrying out separation and purification on a crude extract of fermentation products of the two mutant strains, to give the novel streptovaricins derivative 1-4. The novel streptovaricins derivative of the present invention has good antibacterial effects against methicillin-resistant Staphylococcus aureus, Escherichia coli, and Acinetobacter baumannii, provides a new solution to the issue of antibiotic resistance, and provides a basis for structural modification of streptovaricins antibiotics.
Inventors:
SUN YUHUI (CN)
LIU YUANZHEN (CN)
CHEN XU (CN)
DENG ZIXIN (CN)
LIU YUANZHEN (CN)
CHEN XU (CN)
DENG ZIXIN (CN)
Application Number:
PCT/CN2018/097950
Publication Date:
February 14, 2019
Filing Date:
August 01, 2018
Export Citation:
Assignee:
UNIV WUHAN (CN)
International Classes:
C07D491/18; A61K31/395; A61P31/04; C07D225/08; C07D491/08; C12N1/21; C12P17/18; C12R1/465
Domestic Patent References:
| WO1986005784A1 | 1986-10-09 |
Foreign References:
| CN107540682A | 2018-01-05 | |||
| CN105017267A | 2015-11-04 | |||
| JPS5052073A | 1975-05-09 |
Other References:
SONG, JIAOJIAO ET AL.: "Gene Manipulation System for Streptovaricin Producer Streptomyces Spectabilis NRRL2494", JOURNAL OF XIAMEN UNIVERSITY( NATURAL SCIENCE, vol. 55, no. 1, 31 January 2016 (2016-01-31), pages 60 - 66, XP055570277, ISSN: 0438-0479
RINEHART, K.L.J. ET AL.: "Chemistry of the Streptovaricins. VIII. Structures of Streptovaricins A, B, D, E, F, and G", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 93, no. 23, 31 December 1971 (1971-12-31), pages 6273 - 6274, XP055570295, ISSN: 0002-7863
RINEHART, K.L.J. ET AL.: "Chemistry of the Streptovaricins. VIII. Structures of Streptovaricins A, B, D, E, F, and G", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 93, no. 23, 31 December 1971 (1971-12-31), pages 6273 - 6274, XP055570295, ISSN: 0002-7863
Attorney, Agent or Firm:
WUHAN KEHAO INTELLECTUAL PROPERTY ATTORNEYS (SPECIAL GENERAL PARTNERSHIP) (CN)
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