Title:
RECOMBINANT ESCHERICHIA COLI AND CONSTRUCTION METHOD THEREFOR, AND METHOD FOR SYNTHESIZING 1,5-PENTANEDIAMINE
Document Type and Number:
WIPO Patent Application WO/2024/000368
Kind Code:
A1
Abstract:
The present invention relates to the technical field of genetic engineering, and provides recombinant Escherichia coli and a construction method therefor, and a method for synthesizing 1,5-pentanediamine. The recombinant Escherichia coli contains an optimal lysine decarboxylase mutant CadAP530L/M569V that catalyzes L-lysine to generate 1,5-pentanediamine, a lysine/cadaverine antiporter CadB that facilitates the entry and exit of a substrate/product into or out of a cell, and pyridoxal kinases I and II that provide self-supply of a cofactor PLP. High-yield 1,5-pentanediamine obtained by using the recombinant Escherichia coli as a catalyst has a high molar conversion rate of 98.67%. According to the method for synthesizing 1,5-pentanediamine provided in the present invention, Escherichia coli is used as a catalyst, no additional cofactor PLP needs to be added, no buffer solution is used (pH does not need to be adjusted), and no hydrochloric acid needs to be used for neutralization, such that the time for synthesizing and converting into 1,5-pentanediamine is short, the production costs are low, and an efficient and economical method is provided for industrial production of 1,5-pentanediamine.
Inventors:
RAO ZHIMING (CN)
QIAO ZHINA (CN)
LIU ZUYI (CN)
XU MEIJUAN (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
QIAO ZHINA (CN)
LIU ZUYI (CN)
XU MEIJUAN (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
Application Number:
PCT/CN2022/102717
Publication Date:
January 04, 2024
Filing Date:
June 30, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12N15/31; C12N15/60; C12N15/70; C12P13/00; C12R1/19
Domestic Patent References:
| WO2022001121A1 | 2022-01-06 |
Foreign References:
| CN105316270A | 2016-02-10 | |||
| CN106148373A | 2016-11-23 | |||
| CN113881719A | 2022-01-04 | |||
| CN108138160A | 2018-06-08 | |||
| CN113444713A | 2021-09-28 |
Other References:
MA, WEICHAO ET AL.: "Advances in Cadaverine Bacterial Production and Its Applications", ENGINEERING, vol. 3, no. 3, 15 June 2017 (2017-06-15), XP055603579, ISSN: 2095-8099, DOI: 10.1016/J.ENG.2017.03.012
JIE DENG, GAO ZHEN; GAO HUA; WU QIAOFEN; JIANG CHENGJIAN: "Research Progress of the Biosynthesis of 1,5-Diaminopentane ", GUANGXI SCIENCES, vol. 24, no. 1, 16 January 2017 (2017-01-16), pages 40 - 47, XP093122091
JIE DENG, GAO ZHEN; GAO HUA; WU QIAOFEN; JIANG CHENGJIAN: "Research Progress of the Biosynthesis of 1,5-Diaminopentane ", GUANGXI SCIENCES, vol. 24, no. 1, 16 January 2017 (2017-01-16), pages 40 - 47, XP093122091
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
Download PDF:
Previous Patent: BATTERY CELL, BATTERY, AND ELECTRIC DEVICE
Next Patent: BATTERY CELL, BATTERY, AND ELECTRIC DEVICE
Next Patent: BATTERY CELL, BATTERY, AND ELECTRIC DEVICE