Title:
NUCLEIC ACID LOADING REAGENT AND METHOD FOR DETECTING ADSORPTION CAPACITY OF SEQUENCING CHIP USING SAME
Document Type and Number:
WIPO Patent Application WO/2024/092388
Kind Code:
A1
Abstract:
The present invention provides a nucleic acid loading reagent and a method for detecting the nucleic acid adsorption capacity of a sequencing chip using same. The method comprises at least one of the following conditions: (i) the nucleic acid loading reagent is at a pH value between 3 and 5; (ii) the nucleic acid loading reagent contains metal ions, the metal ions being, for example, from one or more of the following solutions: MgCl 2, CeCl 3, ZnCl 2, MnCl 2, CaCl 2, CuCl 2, and NiCl 2; and (iii) the chip and the nucleic acid loading reagent are co-incubated for 10 min or above. The present invention provides a quality control scheme for the sequencing chip, which is not limited to platforms, simple to operate, low in cost, and high in speed. The present invention provides a scheme capable of quickly optimizing the loading efficiency and loading bias of a nucleic acid library, and provides a reaction reagent capable of effectively improving the loading efficiency and loading bias of a nucleic acid library.
Inventors:
YANG JIN (CN)
XU CHONGJUN (CN)
LUO HAIYAN (CN)
SU QIONG (CN)
XU CHONGJUN (CN)
LUO HAIYAN (CN)
SU QIONG (CN)
Application Number:
PCT/CN2022/128553
Publication Date:
May 10, 2024
Filing Date:
October 31, 2022
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
International Classes:
C12N15/113
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (Shanghai 2, CN)
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