Title:
NOVEL L-AMINO ACID OXIDASE AND METHOD FOR PRODUCING D-AMINO ACID OR DERIVATIVE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/196619
Kind Code:
A1
Abstract:
The present invention can produce an L-amino acid oxidase at a low cost and in a large quantity through a high expression of a recombinant protein, and the like, and provides a novel L-amino acid oxidase (ArtLAAO) having a wide substrate selectivity. This novel L-amino acid oxidase is a protein comprising any one of amino acid sequences (1) to (4): (1) an amino acid sequence including an amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO; 5, and having an L-amino acid oxidase activity; (2) an amino acid sequence including an amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5 with one or more amino acids deleted, substituted or added, and having an L-amino acid oxidase activity; (3) an amino acid sequence including an amino acid sequence having at least 90% amino acid identity with the amino acid sequence represented by SEQ ID NO: 1, and having an L-amino acid oxidase activity; and (4) an amino acid sequence including an amino acid sequence having at least 98% amino acid identity with the amino acid sequence represented by SEQ ID NO: 3 or SEQ ID NO: 5, and having an L-amino acid oxidase activity.
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Inventors:
ITO SOHEI (JP)
NAKANO SHOGO (JP)
MINAMINO YUKI (JP)
NAKANO SHOGO (JP)
MINAMINO YUKI (JP)
Application Number:
PCT/JP2020/013326
Publication Date:
October 01, 2020
Filing Date:
March 25, 2020
Export Citation:
Assignee:
SHIZUOKA PREFECTURAL UNIV CORPORATION (JP)
International Classes:
C12P7/50; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/06; C12N15/53; C12N15/63; C12P13/04; C12P41/00
Foreign References:
| JP2002262890A | 2002-09-17 |
Other References:
NAKANO, SHOGO ET AL.: "Deracemization and Stereoinversion to Aromatic D- Amino Acid Derivatives with Ancestral L-Amino Acid Oxidase", ACS CATALYSIS, vol. 9, no. 11, 27 September 2019 (2019-09-27), pages 10152 - 10158, XP055743590
ANONYMOUS: "SubName: Full=Amine oxidoreductase, flavin-containing {ECO:0000313|EMBL:ERG16445.1};", UNIPROT , 2019.01.16 , ID UIJIG9_9GAMM, VERSION 20, 16 January 2019 (2019-01-16), XP009523674, Retrieved from the Internet [retrieved on 20200605]
ANONYMOUS, UNIPROT, 2018.12.05 , ID A0AOCIMMN3_9GAMM , VERSION 17, 5 December 2018 (2018-12-05), XP009523675, Retrieved from the Internet [retrieved on 20200605]
ANONYMOUS: "SubName: Full=Amine oxidoreductase {ECO:0000313|EMBL:PCK33141.1}; Version 5", UNIPROT, 2018.12.05 , ID A0A2A5JV24_PSE07, VERSION 5, 5 December 2018 (2018-12-05), XP009523672, Retrieved from the Internet [retrieved on 20200605]
NIWA, MASAZUMI; NAKANO, SHOGO; ASANO, YASUHISA; SOHEI, ITO: "Modification of substrate specificity and thermal stabilization of L-arginine oxidase using the ancestral designmethod", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2018 (JSBBA 2018), 2018, pages 168, XP009523723
ANONYMOUS: "SubName: Full=Amine oxidoreductase, flavin-containing {ECO:0000313|EMBL:ERG16445.1};", UNIPROT , 2019.01.16 , ID UIJIG9_9GAMM, VERSION 20, 16 January 2019 (2019-01-16), XP009523674, Retrieved from the Internet
ANONYMOUS, UNIPROT, 2018.12.05 , ID A0AOCIMMN3_9GAMM , VERSION 17, 5 December 2018 (2018-12-05), XP009523675, Retrieved from the Internet
ANONYMOUS: "SubName: Full=Amine oxidoreductase {ECO:0000313|EMBL:PCK33141.1}; Version 5", UNIPROT, 2018.12.05 , ID A0A2A5JV24_PSE07, VERSION 5, 5 December 2018 (2018-12-05), XP009523672, Retrieved from the Internet
NIWA, MASAZUMI; NAKANO, SHOGO; ASANO, YASUHISA; SOHEI, ITO: "Modification of substrate specificity and thermal stabilization of L-arginine oxidase using the ancestral designmethod", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 2018 (JSBBA 2018), 2018, pages 168, XP009523723
Attorney, Agent or Firm:
HORIKOSHI, Somei et al. (JP)
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