Title:
METHODS OF BARCODING NUCLEIC ACIDS FOR DETECTION AND SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2024/050331
Kind Code:
A3
Abstract:
Described and featured herein are methods to barcode nucleic acids for detection and sequencing, particularly at a single cell level. The methods involve application of a barcode template in a compartment with various targets, including nucleic acid fragments, nuclei and/or cells. After clonal amplification within the compartment, the barcode sequence integrates into its target before the compartment is broken so that it will effectively barcode nucleic acid fragments originated from a nucleic acid fragment, a nucleus or a cell clonally. The barcode information can be used for tracking the origin of the fragment, nucleus or cell and be used for haplotype phasing and a variety of single cell-based applications including whole genome sequencing, metagenome sequencing, targeted sequencing, RNA sequencing and immune repertoire sequencing.
Inventors:
CHEN ZHOUTAO (US)
BASSETS IVAN G (US)
BASSETS IVAN G (US)
Application Number:
PCT/US2023/073042
Publication Date:
May 10, 2024
Filing Date:
August 29, 2023
Export Citation:
Assignee:
UNIVERSAL SEQUENCING TECH CORPORATION (US)
International Classes:
C12N15/10; C12Q1/686
Attorney, Agent or Firm:
HUNTER-ENSOR, Melissa (LLPOne International Place, Suite 200, Boston Massachusetts, US)
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