Title:
METHOD FOR PRODUCING POLYPEPTIDE FROM RECOMBINANT FUSION PROTEIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/087761
Kind Code:
A1
Abstract:
The present invention relates to a method for producing a polypeptide from a recombinant fusion protein, and disclosed are a method for producing a polypeptide by means of serially combining multiple protease cleavage sites and polypeptide sequences in a specific order under the assistance of protein chaperones, and the use thereof. The high-tandem number recombinant engineering bacteria constructed in the present invention can efficiently express soluble recombinant proteins without forming inclusion bodies and with less impurity proteins. The fusion protein captured after high-pressure crushing can be quickly digested by an enzyme within 1-2 h to obtain a target polypeptide monomer. The expression system can be used to produce various polypeptides having 10-60 amino acids and/or an isoelectric point range of 2-10. Compared with a single serial connection, the yield of the target polypeptide is greatly improved. The method is more suitable for industrial large-scale production.
Inventors:
LI YAN (CN)
LI JIEPING (CN)
HUANG MEIQUN (CN)
LI JIEPING (CN)
HUANG MEIQUN (CN)
Application Number:
PCT/CN2023/108890
Publication Date:
May 02, 2024
Filing Date:
July 24, 2023
Export Citation:
Assignee:
YANGZHOU AURISCO PHARMACEUTICAL CO LTD (CN)
GUANGDONG ZETA PHARMACEUTICAL CO LTD (CN)
GUANGDONG ZETA PHARMACEUTICAL CO LTD (CN)
International Classes:
C07K19/00; C12N1/21; C12N9/02; C12N15/70; C12R1/19
Foreign References:
| CN115975047A | 2023-04-18 | |||
| CN1587385A | 2005-03-02 | |||
| US20060024778A1 | 2006-02-02 | |||
| CN110128521A | 2019-08-16 | |||
| CN104232666A | 2014-12-24 |
Other References:
DATABASE Protein 2 January 2014 (2014-01-02), "Thioredoxin domain [Ignisphaera aggregans DSM 17230]", XP093161505, Database accession no. ADM28255
DATABASE Protein 9 April 2019 (2019-04-09), "thioredoxin family protein [Hyperthermus butylicus]", XP093161506, Database accession no. WP_011821942
DATABASE Protein 17 July 2013 (2013-07-17), "Thioredoxin domain-containing protein [Pyrolobus fumarii 1A]", XP093161508, Database accession no. AEM38929
DATABASE Protein 8 April 2019 (2019-04-08), "thioredoxin family protein [Pyrodictium occultum]", XP093161514, Database accession no. WP_058370899
DATABASE Protein 15 October 2018 (2018-10-15), "MAG: thiol reductase thioredoxin [Thermoprotei archaeon]", XP093161516, Database accession no. RLE78521
DATABASE Protein 6 June 2013 (2013-06-06), "thioredoxin [Escherichia coli MS 21-1]", XP093161520, Database accession no. EFK18630
DATABASE Protein 9 April 2019 (2019-04-09), "thioredoxin family protein [Hyperthermus butylicus]", XP093161506, Database accession no. WP_011821942
DATABASE Protein 17 July 2013 (2013-07-17), "Thioredoxin domain-containing protein [Pyrolobus fumarii 1A]", XP093161508, Database accession no. AEM38929
DATABASE Protein 8 April 2019 (2019-04-08), "thioredoxin family protein [Pyrodictium occultum]", XP093161514, Database accession no. WP_058370899
DATABASE Protein 15 October 2018 (2018-10-15), "MAG: thiol reductase thioredoxin [Thermoprotei archaeon]", XP093161516, Database accession no. RLE78521
DATABASE Protein 6 June 2013 (2013-06-06), "thioredoxin [Escherichia coli MS 21-1]", XP093161520, Database accession no. EFK18630
Attorney, Agent or Firm:
YANGZHOU AURISCO PHARMACEUTICAL CO., LTD (CN)
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