Title:
METHOD FOR PRODUCING ACTIVE HGF
Document Type and Number:
WIPO Patent Application WO/2024/096038
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing active HGF in an industrially advantageous manner, by expressing HGF α chain and HGF β chain in the same host cell. The present disclosure provides a method for producing active HGF, the method comprising the following steps 1-4. Step 1: A step for preparing DNA encoding an amino acid sequence including HGF α chain and DNA encoding an amino acid sequence including HGF β chain.
Step 2: A step for inserting respective strands of DNA prepared in step 1 into vectors for expressing protein.
Step 3: A step for expressing HGF α chain and HGF β chain both in a host cell by co-introducing the vectors obtained in step 2 into the host cell, and culturing the host cell.
Step 4: A step for recovering active HGF from a culture supernatant after step 3.
Inventors:
KAYANO YOSHIYUKI (JP)
Application Number:
PCT/JP2023/039364
Publication Date:
May 10, 2024
Filing Date:
November 01, 2023
Export Citation:
Assignee:
KRINGLE PHARMA INC (JP)
International Classes:
C12N15/19; C07K14/475; C12N15/67; C12P21/02
Domestic Patent References:
| WO2017159722A1 | 2017-09-21 |
Foreign References:
| CN110607304A | 2019-12-24 | |||
| CN1385533A | 2002-12-18 |
Attorney, Agent or Firm:
IWATANI Ryo (JP)
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