METHOD FOR MEASURING NUMBER OF CELLS OF LACTOCOCCUS LACTIS IN FERMENTATION COMPOSITION CONTAINING LACTOCOCCUS LACTIS AND STREPTOCOCCUS THERMOPHILUS

Title:

METHOD FOR MEASURING NUMBER OF CELLS OF LACTOCOCCUS LACTIS IN FERMENTATION COMPOSITION CONTAINING LACTOCOCCUS LACTIS AND STREPTOCOCCUS THERMOPHILUS

Document Type and Number:

WIPO Patent Application WO/2024/095989

Kind Code:

A1

Abstract:

The present invention addresses the problem of providing: a method for selectively measuring the number of cells of a bacterium capable of activating a plasmacytoid dendritic cell (e.g., a lactic acid bacterium such as a bacterium of strain JCM5805) at low cost and in a simple manner without the need for a skillful technique in a fermentation composition containing the bacterium capable of activating a plasmacytoid dendritic cell (e.g., a lactic acid bacterium such as a bacterium of strain JCM5805) and a thermophilus bacterium; a fermentation composition suitable for the method; and others.　More specifically, in a preferred aspect (i.e., an aspect in which the bacterium capable of activating a plasmacytoid dendritic cell is a bacterium of strain JCM5805), the present invention addresses the problem of providing: a method for selectively measuring the number of cells of a bacterium of strain JCM5805 at low cost and in a simple manner without the need for a skillful technique in a fermentation composition containing the bacterium of strain JCM5805 and a thermophilus bacterium that are similar to each other with respect to morphology such as size or shape; a fermentation composition suitable for the method; and others.　The present invention relates to: a method for measuring the number of cells of a component (A) that is a bacterium capable of activating a plasmacytoid dendritic cell (e.g., Lactococcus lactis subsp. lactis strain JCM5805) in a fermentation composition containing the component (A) and a component (B) that is a bacterium belonging to Streptococcus salivarius subsp. thermophilus, the method comprising a step (1) of adding a diluent solution to the fermentation composition and a step (2) of incubating a diluted solution produced in the step (1) at 35 to 50°C; and others.

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Inventors:

UEDA KAZUMA (JP)
FUJITA JUN (JP)
KUWAHIRA HIDEO (JP)

Application Number:

PCT/JP2023/039175

Publication Date:

May 10, 2024

Filing Date:

October 31, 2023

Export Citation:

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Assignee:

KOIWAI DAIRY PRODUCTS CO LTD (JP)
KIRIN HOLDINGS KK (JP)

International Classes:

C12Q1/06; A23C9/123; C12N1/20

Attorney, Agent or Firm:

HIROTA, Masanori (2-2-19 Akasaka, Minato-k, Tokyo 52, JP)

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