METHOD FOR CULTURING DEHALOCOCCOIDES GENUS BACTERIA AND METHOD FOR PURIFYING CHLORINATED ETHYLENES

WIPO Patent Application WO/2024/075780

A1

Provided is a method for stably mass culturing Dehalococcoides genus bacteria using a keg-like metal pressure-resistant culture vessel.　This method for culturing Dehalococcoides genus bacteria uses a metal pressure-resistant culture vessel to culture Dehalococcoides genus bacteria in a culture broth while maintaining ion concentrations in the range of (a) 1 mM or less of iron-containing ions, (b) 23 μM or less of chromium-containing ions, and (c) 1.5 mM or less of nickel-containing ions.

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YOSHIDA NAOKO (JP)
YAMAZAKI YUJI (JP)
HISADA TAKAYOSHI (JP)

PCT/JP2023/036235

April 11, 2024

October 04, 2023

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TECHNOSURUGA LABORATORY CO LTD (JP)
NAGOYA INST TECH (JP)
TAKENAKA CORP (JP)

C12N1/20; B09C1/10; C02F3/00; C02F3/34

JP2021016368A	2021-02-15
JP2021016369A	2021-02-15
JP2021000031A	2021-01-07

MORITA, YUKI ET AL.: "Mass culture of Dehalococcoides mccartyi strain NIT01 using beer barrels", THE 25TH SYMPOSIUM ON SOIL AND GROUNDWATER CONTAMINATION AND REMEDIATION, vol. 25, 2019, pages 521 - 522
ASAI, MASAKI ET AL.: "Evaluating the Impact of Metal Vessels on the Large-Scale Cultivation of Dehalococcoides", THE 57TH ANNUAL CONFERENCE OF JAPAN SOCIETY ON WATER ENVIRONMENT, vol. 57, 9 March 2023 (2023-03-09), pages 117

HORIKOSHI Somei et al. (JP)

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