Title:
LIGASE FUSION PROTEINS AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/160156
Kind Code:
A1
Abstract:
The present disclosure relates to the field of biotechnology. In particular, provided are a ligase fusion protein and an immobilized ligase comprising the same. Also provided is use of the ligase fusion protein or the immobilized ligase in the preparation of conjugates. Further provided is a process for the preparation of conjugates using a ligase or a ligase unit.
Inventors:
QIN GANG (CN)
LV CAO (CN)
WALTER JOACHIM (DE)
WERNER ROLF (DE)
ZHONG YUNPENG (CN)
LV CAO (CN)
WALTER JOACHIM (DE)
WERNER ROLF (DE)
ZHONG YUNPENG (CN)
Application Number:
PCT/CN2021/074082
Publication Date:
August 04, 2022
Filing Date:
January 28, 2021
Export Citation:
Assignee:
GENEQUANTUM HEALTHCARE SUZHOU CO LTD (CN)
International Classes:
C12N15/62; A61K31/537; C07K4/00
Domestic Patent References:
| WO2015165413A1 | 2015-11-05 | |||
| WO2014140317A2 | 2014-09-18 | |||
| WO2014177042A1 | 2014-11-06 | |||
| WO2017167712A1 | 2017-10-05 | |||
| WO2020072764A1 | 2020-04-09 |
Other References:
HIDEHIKO HIRAKAWA; TOMOAKI HAGA; TERUYUKI NAGAMUNE: "Artificial Protein Complexes for Biocatalysis", TOPICS IN CATALYSIS, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 55, no. 16 - 18, 29 September 2012 (2012-09-29), Ne , pages 1124 - 1137, XP035144370, ISSN: 1572-9028, DOI: 10.1007/s11244-012-9900-5
HOELZEL CONNER A., ZHANG XIN: "Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐Tag Technologies", CHEMBIOCHEM, vol. 21, no. 14, 16 July 2020 (2020-07-16), pages 1935 - 1946, XP055954597, ISSN: 1439-4227, DOI: 10.1002/cbic.202000037
GUIMARAES CARLA P; WITTE MARTIN D; THEILE CHRISTOPHER S; BOZKURT GUNES; KUNDRAT LENKA; BLOM ANNET E M; PLOEGH HIDDE L: "Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 8, no. 9, 29 August 2013 (2013-08-29), GB , pages 1787 - 1799, XP037547548, ISSN: 1754-2189, DOI: 10.1038/nprot.2013.101
KE NA, LANDGRAF DIRK, PAULSSON JOHAN, BERKMEN MEHMET: "Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 198, no. 7, 1 April 2016 (2016-04-01), US , pages 1035 - 1043, XP055954602, ISSN: 0021-9193, DOI: 10.1128/JB.00864-15
LI ANZHANG, ZONGZE SHAO: "Research progress of microbial haloalkane dehalogenase-A review.", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 55, no. 4, 4 April 2015 (2015-04-04), CN , pages 381 - 388, XP055954605, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.20140304
See also references of EP 4196590A4
HOELZEL CONNER A., ZHANG XIN: "Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP‐Tag Technologies", CHEMBIOCHEM, vol. 21, no. 14, 16 July 2020 (2020-07-16), pages 1935 - 1946, XP055954597, ISSN: 1439-4227, DOI: 10.1002/cbic.202000037
GUIMARAES CARLA P; WITTE MARTIN D; THEILE CHRISTOPHER S; BOZKURT GUNES; KUNDRAT LENKA; BLOM ANNET E M; PLOEGH HIDDE L: "Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 8, no. 9, 29 August 2013 (2013-08-29), GB , pages 1787 - 1799, XP037547548, ISSN: 1754-2189, DOI: 10.1038/nprot.2013.101
KE NA, LANDGRAF DIRK, PAULSSON JOHAN, BERKMEN MEHMET: "Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 198, no. 7, 1 April 2016 (2016-04-01), US , pages 1035 - 1043, XP055954602, ISSN: 0021-9193, DOI: 10.1128/JB.00864-15
LI ANZHANG, ZONGZE SHAO: "Research progress of microbial haloalkane dehalogenase-A review.", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 55, no. 4, 4 April 2015 (2015-04-04), CN , pages 381 - 388, XP055954605, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.20140304
See also references of EP 4196590A4
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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