Title:
HYDROGEL-BASED CELL ENCAPSULATION METHOD, A CELL OR CELL-ENCAPSULATING POLYMERIZED MICROGEL AND A SYSTEM THEREOF, AND KIT THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/231834
Kind Code:
A1
Abstract:
Provided are a hydrogel-based cell encapsulation method, a cell-encapsulating polymerized microgel prepared therefrom, and a kit thereof. The hydrogel-based cell encapsulation method can include: (a) providing a cell suspension as an aqueous phase comprising cells, alginate and a culture medium; (b) preparing an emulsion using the cell suspension and an oil phase through a droplet microfluidic device such that one or more of the cells are each encapsulated in at least a portion of the droplets thereby forming an emulsion comprising cell-encapsulating droplets and empty droplets; (c) incubating the emulsion, and then cross-linking alginate in the cell-encapsulating droplets in the presence of a cross-linker to form cell-encapsulating polymerized microgels; and (d) optionally, demulsifying, separating and collecting the cell-encapsulating polymerized microgels.
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Inventors:
YANG MENGSU (CN)
ZHOU XIAOYU (CN)
WANG YUAN (CN)
HOU XUHUA (CN)
ZHOU XIAOYU (CN)
WANG YUAN (CN)
HOU XUHUA (CN)
Application Number:
PCT/CN2023/095763
Publication Date:
December 07, 2023
Filing Date:
May 23, 2023
Export Citation:
Assignee:
UNIV CITY HONG KONG (CN)
International Classes:
C12N5/00; C12N5/071; C12N11/04
Domestic Patent References:
| WO2022013254A1 | 2022-01-20 |
Foreign References:
| US20150352056A1 | 2015-12-10 | |||
| US20190282510A1 | 2019-09-19 | |||
| US20150071997A1 | 2015-03-12 |
Other References:
CHANG-HYUNG CHOI ET AL.: "Generation of monodisperse alginate microbeads and in situ encapsulation of cell in microfluidic device", BIOMEDICAL MICRODEVICES, vol. 9, no. 6, 20 June 2007 (2007-06-20), pages 855 - 862, XP019548920, ISSN: 1387-2176, DOI: 10.1007/s10544-007-9098-7
LILITH M CABALLERO AGUILAR ET AL.: "Formation of alginate microspheres prepared by optimized microfluidics parameters for high encapsulation of bioactive molecules", JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 587, no. 2021, 13 December 2020 (2020-12-13), pages 240 - 251, XP086473610, ISSN: 0021-9797, DOI: 10.1016/j.jcis.2020.12.026
LILITH M CABALLERO AGUILAR ET AL.: "Formation of alginate microspheres prepared by optimized microfluidics parameters for high encapsulation of bioactive molecules", JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 587, no. 2021, 13 December 2020 (2020-12-13), pages 240 - 251, XP086473610, ISSN: 0021-9797, DOI: 10.1016/j.jcis.2020.12.026
Attorney, Agent or Firm:
BEIJING PAT SF INTELLECTUAL PROPERTY AGENCY CO. LTD (CN)
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