Title:
GLUCOSAMINE SALT PRODUCED BY MEANS OF ENZYMATIC METHOD, AND PURIFICATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/248697
Kind Code:
A1
Abstract:
Provided are a glucosamine salt produced by means of an enzymatic method, and a purification method therefor. According to the enzymatic method, N-acetylglucosamine is taken as a raw material and is subjected to deacetylase hydrolysis to obtain glucosamine and acetic acid; and then same are separated by means of eluting a cation exchange column with an acid solution to obtain glucosamine salt; moreover, a by-product, i.e. sodium acetate, is obtained by means of anion exchange recovery. The obtained glucosamine salt is concentrated, crystallized, de-colored and dried to obtain a high-purity glucosamine salt crystal. By means of combining a cyclic utilization process of enzymes, a cyclic recovery process of residual substrates and a recovery process of acetic acid, the enzymes are cyclically utilized, such that the residual substrates are sufficiently converted and resource utilization of the acetic acid is realized, while the conversion rate of N-acetylglucosamine and the total yield of glucosamine salt products are increased; furthermore, by means of operation conditions at room temperature, the loss rate of resin is low and the output of hydrochloric acid waste liquid is very little, thereby realizing the economic benefits of energy conservation and consumption reduction and the effects of being environmentally friendly and safe.
Inventors:
DING CHUNHUA (CN)
ZHANG WENJIE (CN)
ZHANG WENJIE (CN)
Application Number:
PCT/CN2020/111623
Publication Date:
December 16, 2021
Filing Date:
August 27, 2020
Export Citation:
Assignee:
JIANGSU HEVI BIOTECH CO LTD (CN)
BIOKEY PTE LTD (SG)
BIOKEY PTE LTD (SG)
International Classes:
C12P19/26; C07H1/06; C07H5/06
Foreign References:
| CN101365785A | 2009-02-11 | |||
| CN111518857A | 2020-08-11 | |||
| CN109666663A | 2019-04-23 | |||
| US20060210582A1 | 2006-09-21 | |||
| CN110714042A | 2020-01-21 | |||
| CN107267579A | 2017-10-20 |
Other References:
WANG, CHAO: "A study on recovery and purification conditions of glucosamine hydrochloride mother liquor", BIOTECH WORLD, 31 December 2016 (2016-12-31), pages 243, XP055878168
JAMES S. DAVIES; DAVID COOMBES; CHRISTOPHER R. HORNE; F. GRANT PEARCE; ROSMARIE FRIEMANN; RACHEL A. NORTH; RENWICK C. J. DOBSON: "Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus", FEBS LETTERS, vol. 593, no. 1, 26 November 2018 (2018-11-26), NL , pages 52 - 66, XP071256661, ISSN: 0014-5793, DOI: 10.1002/1873-3468.13289
JAMES S. DAVIES; DAVID COOMBES; CHRISTOPHER R. HORNE; F. GRANT PEARCE; ROSMARIE FRIEMANN; RACHEL A. NORTH; RENWICK C. J. DOBSON: "Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus", FEBS LETTERS, vol. 593, no. 1, 26 November 2018 (2018-11-26), NL , pages 52 - 66, XP071256661, ISSN: 0014-5793, DOI: 10.1002/1873-3468.13289
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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