Title:
FLUOROACETATE DEHALOGENASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/120134
Kind Code:
A1
Abstract:
Disclosed in the present invention is a fluoroacetate dehalogenase mutant, a sequence of the fluoroacetate dehalogenase mutant comprising a mutated sequence having an amino acid residue H at position 155 and/or an amino acid residue W at position 156, as shown in SEQ ID NO: 1; the fluoroacetate dehalogenase mutant has activity catalyzing bromination of a substrate, particularly a 2-bromobutyric acid substrate. Also provided in the present invention is an application of said fluoroacetate dehalogenase mutant in the preparation of (R)-2-bromobutyric acid and/or (R)-2-hydroxybutyric acid. When using the fluoroacetate dehalogenase mutant of the present invention to prepare (R)-2-bromobutyric acid, production costs are low and stereoselectivity is high, facilitating industrialized production.
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Inventors:
CHENG ZHANBING (CN)
DING SHAONAN (CN)
XU YANBING (CN)
HUANG YAO (CN)
DING SHAONAN (CN)
XU YANBING (CN)
HUANG YAO (CN)
Application Number:
PCT/CN2019/126785
Publication Date:
June 24, 2021
Filing Date:
December 19, 2019
Export Citation:
Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/14; C12N15/55; C12P7/52; C12P41/00; C12R1/38
Foreign References:
| CN109468347A | 2019-03-15 |
Other References:
CHAN PETER W. Y., YAKUNIN ALEXANDER F., EDWARDS ELIZABETH A., PAI EMIL F.: "Mapping the Reaction Coordinates of Enzymatic Defluorination", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 133, no. 19, 18 May 2011 (2011-05-18), pages 7461 - 7468, XP055822102, ISSN: 0002-7863, DOI: 10.1021/ja200277d
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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