Title:
EXPRESSION VECTOR OF BIOSYNTHETIC METABOLIC PATHWAY GENE OF 2-PIPERIDONE AND SYNTHESIS METHOD FOR 2-PIPERIDONE
Document Type and Number:
WIPO Patent Application WO/2023/097668
Kind Code:
A1
Abstract:
Provided are an expression vector of a biosynthetic metabolic pathway gene of 2-piperidone and a synthesis method for 2-piperidone. The expression vector comprises a first promoter, and a davA-davB gene or a davB-davA gene sequentially inserted at downstream of the first promoter, a Pb promoter or a mutant thereof, an act gene or an orf26 gene or a caiC gene, and a ChnR gene or a mutant thereof. The expression vector is further transferred into corynebacterium glutamicum having a high yield of lysine, and fermentation culture is carried out to efficiently synthesize 2-piperidone.
Inventors:
LUO XIAOZHOU (CN)
ZHAO XIXI (CN)
FENG TINGYE (CN)
LU HUAN (CN)
ZHAO XIXI (CN)
FENG TINGYE (CN)
LU HUAN (CN)
Application Number:
PCT/CN2021/135394
Publication Date:
June 08, 2023
Filing Date:
December 03, 2021
Export Citation:
Assignee:
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C12N15/77; C12N1/21; C12N15/11; C12P1/04
Foreign References:
| CN107438667A | 2017-12-05 | |||
| CN113304700A | 2021-08-27 | |||
| US20200263211A1 | 2020-08-20 |
Other References:
RUOSHI LUO, YANG XIZHI, CHENG JIE, XU YANQIN, ZHOU ZHE: "Production of 5-aminovaleric Acid, a Bio-based Plastic Monomer by Metabolic Engineering of Escherichia Coli", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, NANJING UNIVERSITY OF TECHNOLOGY, CN, vol. 18, no. 1, 31 January 2020 (2020-01-31), CN , pages 126 - 132, XP093069606, ISSN: 1672-3678, DOI: 10.3969/j.issn.1672-3678.2020.01.015
SHIN JAE HO, PARK SEOK HYUN, OH YOUNG HOON, CHOI JAE WOONG, LEE MOON HEE, CHO JAE SUNG, JEONG KI JUN, JOO JEONG CHAN, YU JAMES, PA: "Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid", MICROBIAL CELL FACTORIES, vol. 15, no. 1, 7 October 2016 (2016-10-07), pages 174, XP093069609, DOI: 10.1186/s12934-016-0566-8
SHIN JAE HO, PARK SEOK HYUN, OH YOUNG HOON, CHOI JAE WOONG, LEE MOON HEE, CHO JAE SUNG, JEONG KI JUN, JOO JEONG CHAN, YU JAMES, PA: "Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid", MICROBIAL CELL FACTORIES, vol. 15, no. 1, 7 October 2016 (2016-10-07), pages 174, XP093069609, DOI: 10.1186/s12934-016-0566-8
Attorney, Agent or Firm:
BEIJING CHENGHUI LAW FIRM (CN)
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