Title:
CORYNEBACTERIUM SP. MICROBIAL VARIANT PRODUCING L-GLUTAMIC-ACID, AND METHOD FOR PRODUCING L-GLUTAMIC ACID BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2024/014697
Kind Code:
A1
Abstract:
The present invention relates to a Corynebacterium sp. microbial variant producing L-glutamic acid and a method for producing L-glutamic acid by using same, and, more particularly, to a novel variant of FAD-dependent oxidoreductase involved in the L-glutamic acid biosynthetic pathway, a polynucleotide therefor, and a microorganism transformed therewith, and a method of producing L-glutamic acid by using same. The FAD-dependent oxidoreductase variant, according to the present invention, has one or more amino acids substituted in the amino acid sequence constituting the FAD-dependent oxidoreductase, and consequently, the activity of the enzyme is altered in such a way that a recombinant microorganism comprising the variant can efficiently produce L-glutamic acid.
Inventors:
LEE SUN HEE (KR)
KIM HYUN HO (KR)
KIM DONG HYUN (KR)
KIM HYUN SOOK (KR)
PARK SEOK HYUN (KR)
PARK JOON HYUN (KR)
KIM HYUN HO (KR)
KIM DONG HYUN (KR)
KIM HYUN SOOK (KR)
PARK SEOK HYUN (KR)
PARK JOON HYUN (KR)
Application Number:
PCT/KR2023/007160
Publication Date:
January 18, 2024
Filing Date:
May 25, 2023
Export Citation:
Assignee:
DAESANG CORP (KR)
International Classes:
C12N9/02; C12N15/77; C12P13/14
Foreign References:
| KR100837844B1 | 2008-06-13 | |||
| KR102269639B1 | 2021-06-25 | |||
| JP2014036576A | 2014-02-27 |
Other References:
DATABASE Protein 20 May 2022 (2022-05-20), ANONYMOUS: "FAD-dependent oxidoreductase [Corynebacterium glutamicum]", XP093128413, retrieved from NCBI Database accession no. WP_216312814.1
VAN DEN HEUVEL R. H. H., CURTI B., VANONI M. A., MATTEVI A.: "Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate", CMLS CELLULAR AND MOLECULAR LIFE SCIENCES., BIRKHAUSER VERLAG, HEIDELBERG., DE, vol. 61, no. 6, 1 March 2004 (2004-03-01), DE , pages 669 - 681, XP093128416, ISSN: 1420-682X, DOI: 10.1007/s00018-003-3316-0
VAN DEN HEUVEL R. H. H., CURTI B., VANONI M. A., MATTEVI A.: "Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate", CMLS CELLULAR AND MOLECULAR LIFE SCIENCES., BIRKHAUSER VERLAG, HEIDELBERG., DE, vol. 61, no. 6, 1 March 2004 (2004-03-01), DE , pages 669 - 681, XP093128416, ISSN: 1420-682X, DOI: 10.1007/s00018-003-3316-0
Attorney, Agent or Firm:
PARK, Hyuk (KR)
Download PDF: