Title:
CHEMICALLY-INDUCED OSTEOGENIC CELLS AND USE THEREOF IN DISEASE MODELING, AND SCAFFOLD FOR BONE GRAFTING
Document Type and Number:
WIPO Patent Application WO/2024/053957
Kind Code:
A1
Abstract:
The present invention provides a medium composition for direct differentiation of osteogenic cells and a method for direct differentiation of osteogenic cells, which induce osteogenic cells from somatic cells, the composition comprising, as active ingredients, chemicals, that is, a TGF-β receptor inhibitor (RepSox), a cAMP signaling activator (Forskolin) and an epithelial Na+ channel inhibitor (Phenamil). It has been identified that a composition comprising osteogenic cells prepared through the method is effective in treating or preventing osteogenesis imperfecta, which is a prototypical bone disease, and Proteus syndrome, and thus can be effectively used as a composition for preventing or treating bone diseases.
Inventors:
KIM HAE WON (KR)
LEE JUNG HWAN (KR)
YOON JI YOUNG (KR)
LEE JUNG HWAN (KR)
YOON JI YOUNG (KR)
Application Number:
PCT/KR2023/013146
Publication Date:
March 14, 2024
Filing Date:
September 04, 2023
Export Citation:
Assignee:
UNIV DANKOOK CHEONAN CAMPUS IND ACADEMIC COOPERATION FOUNDATION (KR)
International Classes:
C12N5/077; A23L33/10; A61K35/32; A61L27/56; A61P19/00
Foreign References:
| KR20180096538A | 2018-08-29 | |||
| KR20180073631A | 2018-07-02 | |||
| KR20160145174A | 2016-12-19 | |||
| KR20200129274A | 2020-11-18 | |||
| KR20130085752A | 2013-07-30 |
Other References:
K. W. PARK, H. WAKI, W.-K. KIM, B. S. J. DAVIES, S. G. YOUNG, F. PARHAMI, P. TONTONOZ: "The Small Molecule Phenamil Induces Osteoblast Differentiation and Mineralization", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, US, vol. 29, no. 14, 15 July 2009 (2009-07-15), US , pages 3905 - 3914, XP055452467, ISSN: 0270-7306, DOI: 10.1128/MCB.00002-09
BAR-NUR ORI, GERLI MATTIA F.M., DI STEFANO BRUNO, ALMADA ALBERT E., GALVIN AMY, COFFEY AMY, HUEBNER AARON J., FEIGE PETER, VERHEUL: "Direct Reprogramming of Mouse Fibroblasts into Functional Skeletal Muscle Progenitors", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 10, no. 5, 1 May 2018 (2018-05-01), United States , pages 1505 - 1521, XP093147876, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.04.009
YOON JI-YOUNG, MANDAKHBAYAR NANDIN, HYUN JEONGEUN, YOON DONG SUK, PATEL KAPIL D., KANG KEUNSOO, SHIM HO-SHUP, LEE HAE-HYOUNG, LEE : "Chemically-induced osteogenic cells for bone tissue engineering and disease modeling", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 289, 1 October 2022 (2022-10-01), AMSTERDAM, NL , pages 121792, XP093147880, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2022.121792
BAR-NUR ORI, GERLI MATTIA F.M., DI STEFANO BRUNO, ALMADA ALBERT E., GALVIN AMY, COFFEY AMY, HUEBNER AARON J., FEIGE PETER, VERHEUL: "Direct Reprogramming of Mouse Fibroblasts into Functional Skeletal Muscle Progenitors", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 10, no. 5, 1 May 2018 (2018-05-01), United States , pages 1505 - 1521, XP093147876, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.04.009
YOON JI-YOUNG, MANDAKHBAYAR NANDIN, HYUN JEONGEUN, YOON DONG SUK, PATEL KAPIL D., KANG KEUNSOO, SHIM HO-SHUP, LEE HAE-HYOUNG, LEE : "Chemically-induced osteogenic cells for bone tissue engineering and disease modeling", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 289, 1 October 2022 (2022-10-01), AMSTERDAM, NL , pages 121792, XP093147880, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2022.121792
Attorney, Agent or Firm:
BLT PATENT & LAW FIRM (KR)
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