Title:
BACTERIAL TRYPTOPHAN-5-HYDROXYLASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/093260
Kind Code:
A1
Abstract:
Provided are a bacterial tryptophan-5-hydroxylase and use thereof. The bacterial tryptophan-5-hydroxylase comprises the amino acid sequence set forth in SEQ ID NO. 1, SEQ ID NO. 3, or SEQ ID NO. 5, or an amino acid sequence having 50% or higher homology and equivalent functionality to any one of the listed amino acid sequences. A novel bacterial tryptophan-5-hydroxylase family with protoheme as a cofactor capable of catalyzing the production of 5-hydroxytryptophan from tryptophan in vivo or in vitro is identified. The enzyme can be applied to catalyzing the preparation of 5-hydroxytryptophan from tryptophan or applied to the preparation of melatonin. An engineered strain expressing heterologous tryptophan-5-hydroxylase, methyltransferase and decarboxylase genes without tryptophan dioxygenase gene is used as the fermentation strain. Melatonin is specifically produced by means of microorganism fermentation. The method is environment-friendly, cost-efficient, efficient, and thus prospective in industrial production.
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Inventors:
DU YILING (CN)
SHI XINJIE (CN)
LI HU (CN)
ZHAO GUIYUN (CN)
SHI XINJIE (CN)
LI HU (CN)
ZHAO GUIYUN (CN)
Application Number:
PCT/CN2023/101322
Publication Date:
May 10, 2024
Filing Date:
June 20, 2023
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12P13/22; C12N1/21; C12N15/76; C12P17/10
Attorney, Agent or Firm:
HANGZHOU TIANQIN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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